ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

Weihong Xie; Chenqiu Zhang; Zheyu Wang; Hui Chen; Tonghui Gu; Tao Zhou; Yaoxing Wu; Fan Xia; Min Li; Jun Wang; Renjie Jiao; Jun Cui; Shouheng Jin

doi:10.1080/15548627.2023.2233846

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

Autophagy. 2023 Nov;19(11):2853-2868. doi: 10.1080/15548627.2023.2233846. Epub 2023 Jul 11.

Authors

Weihong Xie¹, Chenqiu Zhang², Zheyu Wang², Hui Chen¹, Tonghui Gu², Tao Zhou², Yaoxing Wu², Fan Xia², Min Li³, Jun Wang⁴, Renjie Jiao¹, Jun Cui², Shouheng Jin²

Affiliations

¹ Sino-French Hoffmann Institute, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong, China.
² Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.
³ School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, China.
⁴ Department of Pediatric Surgery, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangdong Provincial Clinical Research Center for Child Health, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

PMID: 37434364
PMCID: PMC10549193 (available on 2024-07-11)
DOI: 10.1080/15548627.2023.2233846

Abstract

Baf A₁: bafilomycin A₁; GABARAP: GABA type A receptor-associated protein; GFP: green fluorescent protein; IFN: interferon; IKBKE/IKKi: inhibitor of nuclear factor kappa B kinase subunit epsilon; IRF3: interferon regulatory factor 3; ISG: interferon-stimulated gene; ISRE: IFN-stimulated response element; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MAVS: mitochondrial antiviral signaling protein; MOI: multiplicity of infection; PAMPs: pathogen-associated molecule patterns; RIGI/DDX58: RNA sensor RIG-I; SeV: Sendai virus; siRNA: small interfering RNA; TBK1: TANK binding kinase 1; WT: wild-type; VSV: vesicular stomatitis virus.

Keywords: ATG4B; Antiviral immune response; LC3-interacting region; TBK1; selective autophagy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antiviral Agents*
Autophagy
Humans
Immunity, Innate
Interferons
Mice
Signal Transduction*

Substances

Antiviral Agents
ATG4B protein, human
Atg4b protein, mouse
GABARAP protein, human
GABARAP protein, mouse
Interferons
TBK1 protein, human
Tbk1 protein, mouse

Grants and funding

This work was supported by the National Natural Science Foundation of China (32170876, 31970700, 32270922, 92042303, 31970538 and 31870862), Science and Technology Planning Project of Guangzhou, China (201907010038), Guangdong Basic and Applied Basic Research Foundation (2020B1515120090), and the Fundamental Research Funds for the Central Universities, Sun Yat-sen University (22qntd2601 and 23lgbj012), Plan on enhancing scientific research in GMU (02-410-2302269XM), National Key R&D Program of China (2021YFA0805800 and 2020YFA0803202), the Guangzhou Medical University Discipline Construction Funds (Basic Medicine) (JCXKJS2022A02), the 111 Project (D18010), the Local Innovative and Research Teams Project of Guangdong Perl River Talents Program (2017BT01S155).