Deep Eutectic Solvent Stir Bar Sorptive Extraction: A Rapid Microextraction Technique for the Determination of Vitamin D3 by Spectrophotometry

Sayyed Hossein Hashemi; Massoud Kaykhaii; Ahmad Jamali Keikha; Leila Raisi

doi:10.1021/acsomega.3c01670

Deep Eutectic Solvent Stir Bar Sorptive Extraction: A Rapid Microextraction Technique for the Determination of Vitamin D₃ by Spectrophotometry

ACS Omega. 2023 Jun 23;8(26):23643-23650. doi: 10.1021/acsomega.3c01670. eCollection 2023 Jul 4.

Authors

Sayyed Hossein Hashemi¹, Massoud Kaykhaii², Ahmad Jamali Keikha³, Leila Raisi¹

Affiliations

¹ Department of Marine Chemistry, Faculty of Marine Science, Chabahar Maritime University, Chabahar, Iran.
² Department of Process Engineering and Chemical Technology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 St., Gdansk 80-233, Poland.
³ Department of Mechanical Engineering, Faculty of Marine Engineering, Chabahar Maritime University, Chabahar, Iran.

Abstract

In this paper, a new microextraction method, named deep eutectic solvent stir bar sorptive extraction (DES-SBSE), is described that utilizes a hydrophobic DES (hDES) as a coating for SBSE. As a model, vitamin D₃ was extracted efficiently by this technique from different real samples before its spectrophotometric determination. A conventional magnet was encapsulated inside a glass bar (1.0 cm × 2 mm) and coated by the hDES consisting of tetrabutylammonium chloride and heptadecanoic acid (mole ratio 1:2). Parameters affecting microextraction were studied and optimized by one-variable-at-a-time, central composite design, and Box-Behnken design methods. Under the optimum conditions, the detection limit of 0.08 μg L^-1 could be reached. The linear range of the method was between 0.5 and 1000.0 μg L^-1 for the analyte. Precision for intraday repeatability and interday reproducibility of the method was better than 3.1 and 4.2, respectively. A single stir bar could be used for at least 50 successive extractions and the batch-to-batch reproducibility of SB coated with hDES was 4.5%.