Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Gael Tchokomeni Siwe; Marguerite Kamdem Simo; Rukesh Maharjan; Andre Perfusion Amang; Christophe Mezui; Paul Vernyuy Tan

doi:10.1099/acmi.0.000432

Metabolomic profiling and bactericidal effect of Polyalthia longifolia (Sonn.) Twaites. stem bark against methicillin-resistant Staphylococcus aureus

Access Microbiol. 2023 Jun 29;5(6):acmi000432. doi: 10.1099/acmi.0.000432. eCollection 2023.

Authors

Gael Tchokomeni Siwe¹, Marguerite Kamdem Simo², Rukesh Maharjan³, Andre Perfusion Amang², Christophe Mezui⁴, Paul Vernyuy Tan¹

Affiliations

¹ Department of Animal Biology & Physiology, Faculty of Sciences, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
² Department of Biological Sciences, Faculty of Sciences, University of Maroua, P.O. Box 814, Maroua, Cameroon.
³ H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, P.O. Box 75270, Karachi, Pakistan.
⁴ Department of Animal Biology, Higher Teacher Training College, ENS, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon.

Abstract

Objective. The present study was carried out to establish the chemical profile of the methanolic extract of Polyalthia longifolia stem bark and investigate its antibacterial property against some human pathogenic bacteria. Methods. The extract was analysed using liquid and gas chromatography coupled to mass spectrometry. Antibacterial activity of P. longifolia extract against some human pathogenic bacteria was screened using the AlamarBlue method, and MIC and MBC were determined. Results and Conclusion. Liquid chromatography-mass spectrometry (LC-MS) revealed the presence of 21 compounds among which 12 were identified. Gas chromatography-mass spectrometry (GC-MS) allowed identification of 26 compounds, the three major ones being the following: cis vaccenic acid (17.79 %), 3-ethyl-3-hydroxyandrostan-17-one (13.80 %) and copaiferic acid B (12.82 %). P. longifolia extract was active against Gram-positive bacteria with MIC ranging from 1 to 2 mg ml^-1 and MBC from 2 to 6 mg ml^-1. This study demonstrated the bactericidal effect of the methanolic extract of Polyalthia longifolia stem bark against some human pathogenic bacteria, including methicillin-resistant S. aureus . This effect could be related to the presence in the extract of a broad diversity of well-known compounds with established pharmacological properties. These results support the ethnomedicinal use of P. longifolia stem bark in Cameroon for the management of methicillin-resistant S. aureus (MRSA)-related infections.

Keywords: GC-MS; LC-MS; MRSA; Polyalthia longifolia; bactericidal activity.