Application of DNA Nanotweezers in biosensing: Nanoarchitectonics and advanced challenges

Biosens Bioelectron. 2023 Oct 1:237:115445. doi: 10.1016/j.bios.2023.115445. Epub 2023 Jul 3.

Abstract

Deoxyribonucleic acid (DNA) is a carrier of genetic information. DNA hybridization is characterized by predictability, diversity, and specificity owing to the strict complementary base-pairing assembly mode, which stimulates the use of DNA to build a variety of nanomachines, including DNA tweezers, motors, walkers, and robots. DNA nanomachines have become prevalent for signal amplification and transformation in the field of biosensing, providing a new method for constructing highly sensitive sensing analysis strategies. DNA tweezers have exhibited unique advantages in biosensing applications owing to their simple structures and fast responses. The two-state conformation of DNA tweezers, the open and closed states, enable them to open and close autonomously after stimulation, thus facilitating the quick detection of corresponding signal changes of different targets. This review discusses the recent progress in the application of DNA nanotweezers in the field of biosensing, and the trends in their development for application in the field of biosensing are summarized.

Keywords: Cell imaging; Electrochemistry; Fluorescence; Framework nucleic acid; In vitro detection.

Publication types

  • Review

MeSH terms

  • Biosensing Techniques* / methods
  • DNA* / chemistry
  • Nucleic Acid Conformation
  • Nucleic Acid Hybridization

Substances

  • DNA