Overexpression of 2-Cys Peroxiredoxin alleviates the NaHCO3 stress-induced photoinhibition and reactive oxygen species damage of tobacco

Jiechen Wang; Jiaqi Song; Hongling Qi; Hongjiao Zhang; Lu Wang; Hongbo Zhang; Congcong Cui; Guangxin Ji; Salman Muhammad; Guangyu Sun; Zhiru Xu; Huihui Zhang

doi:10.1016/j.plaphy.2023.107876

Overexpression of 2-Cys Peroxiredoxin alleviates the NaHCO₃ stress-induced photoinhibition and reactive oxygen species damage of tobacco

Plant Physiol Biochem. 2023 Aug:201:107876. doi: 10.1016/j.plaphy.2023.107876. Epub 2023 Jul 1.

Authors

Affiliations

¹ Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
² College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, 157011, China.
³ College of Life Sciences, Northeast Forestry University, Harbin, 150040, China. Electronic address: xuzhiru2003@126.com.
⁴ Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China. Electronic address: zhang_hh@nefu.edu.cn.

PMID: 37413942
DOI: 10.1016/j.plaphy.2023.107876

Abstract

Plant 2-cysteine peroxiredoxin (2-Cys Prx) is a mercaptan peroxidase localized in chloroplasts and has unique catalytic properties. To explore the salt stress tolerance mechanisms of 2-Cys Prx in plants, we analyzed the effects of overexpressing the 2-CysPrx gene on the physiological and biochemical metabolic processes of tobacco under NaHCO₃ stress through joint physiological and transcriptomic analysis. These parameters included growth phenotype, chlorophyll, photosynthesis, and antioxidant system. After NaHCO₃ stress treatment, a total of 5360 differentially expressed genes (DEGs) were identified in 2-Cysprx overexpressed (OE) plants, and the number of DEGs was significantly lower than 14558 in wild-type (WT) plants. KEGG enrichment analysis showed that DEGs were mainly enriched in photosynthetic pathways, photosynthetic antenna proteins, and porphyrin and chlorophyll metabolism. Overexpressing 2-CysPrx significantly reduced the growth inhibition of tobacco induced by NaHCO₃ stress, alleviating the down-regulation of the DEGs related to chlorophyll synthesis, photosynthetic electron transport and the Calvin cycle and the up-regulation of those related to chlorophyll degradation. In addition, it also interacted with other redox systems such as thioredoxins (Trxs) and the NADPH-dependent Trx reductase C (NTRC), and mediated the positive regulation of the activities of antioxidant enzymes such as peroxidase (POD) and catalase (CAT) and the expression of related genes, thereby reducing the accumulation of superoxide anion (O_2·^-), hydrogen peroxide (H₂O₂) and malondialdehyde (MDA). In conclusion, 2-CysPrx overexpression could alleviate the NaHCO₃ stress-induced photoinhibition and oxidative damage by regulating chlorophyll metabolism, promoting photosynthesis and participating in the regulation of antioxidant enzymes, and thus improve the ability of plants to resist salt stress damage.

Keywords: 2-Cys Prx; Antioxidant system; NaHCO(3) stress; Photosynthesis; Reactive oxygen; Tobacco.

MeSH terms

Antioxidants* / metabolism
Chlorophyll
Cysteine / metabolism
Hydrogen Peroxide / metabolism
Nicotiana / genetics
Nicotiana / metabolism
Oxidoreductases / metabolism
Peroxidase / metabolism
Peroxiredoxins* / genetics
Peroxiredoxins* / metabolism
Peroxiredoxins* / pharmacology
Photosynthesis
Reactive Oxygen Species / metabolism

Substances

Reactive Oxygen Species
Antioxidants
Peroxiredoxins
Hydrogen Peroxide
Cysteine
Oxidoreductases
Peroxidase
Chlorophyll