Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Materials

Joseph Ready; Callie Seaman

doi:10.1007/978-1-0716-3319-9_11

Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Materials

Methods Mol Biol. 2023:2688:123-133. doi: 10.1007/978-1-0716-3319-9_11.

Authors

Joseph Ready¹, Callie Seaman²

Affiliations

¹ Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK. jr8158@exchange.shu.ac.uk.
² Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.

PMID: 37410289
DOI: 10.1007/978-1-0716-3319-9_11

Abstract

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a sensitive technique which enables fast, spatially resolved analysis of elements at trace concentration levels in a range of solid sample types, including plant materials. Within this chapter, we describe how to prepare leaf material and seeds for elemental distribution imaging, how to embed material in gelatin and epoxy resin, how to produce matrix-matched reference materials, and how to optimize laser ablation methods.

Keywords: Elemental distribution; Laser ablation inductively coupled plasma mass spectrometry; Matrix-matched; NIST 612 glass standard; Plant analysis.

MeSH terms

Food
Laser Therapy*
Lasers
Mass Spectrometry / methods
Spectrum Analysis