MALDI and Trace Metal Analysis in Age-Related Macular Degeneration

Joshua Millar; Susan Campbell; Catherine Duckett; Sarah Doyle; Laura M Cole

doi:10.1007/978-1-0716-3319-9_1

MALDI and Trace Metal Analysis in Age-Related Macular Degeneration

Methods Mol Biol. 2023:2688:1-13. doi: 10.1007/978-1-0716-3319-9_1.

Authors

Joshua Millar¹, Susan Campbell¹, Catherine Duckett¹, Sarah Doyle², Laura M Cole³

Affiliations

¹ Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
² Immunobiology Research Group, Department of Clinical Medicine, Trinity College Institute of Neuro-science (TCIN), School of Medicine, Trinity College Dublin (TCD), Dublin, Ireland.
³ Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK. L.Cole@shu.ac.uk.

PMID: 37410279
DOI: 10.1007/978-1-0716-3319-9_1

Abstract

Age-related macular degeneration (AMD) remains one of the most prevalent causes of blindness throughout the world. Key to prevention of AMD is furthering the understanding of its pathology. In recent years, both the proteins within the innate immune system and essential and non-essential metals have been implicated in the pathology of AMD. Herein, a multidisciplinary and multimodal methodology has been taken to further our understanding of the role of the innate immune proteins and the essential metals within mouse ocular tissue.

Keywords: Copper; Innate immune system; LA-ICP-MSI; MALDI-MSI; Metals; SARM1; Zinc.

MeSH terms

Animals
Macular Degeneration* / metabolism
Metals
Mice
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Trace Elements* / metabolism

Substances

Trace Elements
Metals