The potent BECN2-ATG14 coiled-coil interaction is selectively critical for endolysosomal degradation of GPRASP1/GASP1-associated GPCRs

Xianxiu Qiu; Na Li; Qifan Yang; Shuai Wu; Xiaohua Li; Xuehua Pan; Soh Yamamoto; Xiaozhe Zhang; Jincheng Zeng; Jiahao Liao; Congcong He; Renxiao Wang; Yanxiang Zhao

doi:10.1080/15548627.2023.2233872

The potent BECN2-ATG14 coiled-coil interaction is selectively critical for endolysosomal degradation of GPRASP1/GASP1-associated GPCRs

Autophagy. 2023 Nov;19(11):2884-2898. doi: 10.1080/15548627.2023.2233872. Epub 2023 Jul 13.

Authors

Xianxiu Qiu^{1

2}, Na Li^{1

3}, Qifan Yang⁴, Shuai Wu⁵, Xiaohua Li⁶, Xuehua Pan⁷, Soh Yamamoto⁸, Xiaozhe Zhang^{1

3}, Jincheng Zeng², Jiahao Liao², Congcong He⁸, Renxiao Wang⁹, Yanxiang Zhao^{1

3}

Affiliations

¹ Department of Applied Biology and Chemical Technology, State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hong Kong, P. R. China.
² Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, the First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan, P.R. China.
³ The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, P.R. China.
⁴ State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, P.R. China.
⁵ Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
⁶ Department of Research and Development, Shenzhen Shiningbiotek Co. Ltd, Shenzhen, P. R. China.
⁷ Shenzhen Pengcheng Biopharm Co. Ltd, Shenzhen, P.R. China.
⁸ Department of Cell and Molecular Biology, Feingberg School of Medicine, Northwestern University, Chicago, IL, USA.
⁹ Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai, People's Republic of China.

PMID: 37409929
PMCID: PMC10549190 (available on 2024-07-13)
DOI: 10.1080/15548627.2023.2233872

Abstract

AMBRA1 autophagy and beclin 1 regulator 1; ATG14 autophagy related 14; ATG5 autophagy related 5; ATG7 autophagy related 7; BECN1 beclin 1; BECN2 beclin 2; CC coiled-coil; CQ chloroquine CNR1/CB1R cannabinoid receptor 1 DAPI 4',6-diamidino-2-phenylindole; dCCD delete CCD; DRD2/D2R dopamine receptor D2 GPRASP1/GASP1 G protein-coupled receptor associated sorting protein 1 GPCR G-protein coupled receptor; ITC isothermal titration calorimetry; IP immunoprecipitation; KD knockdown; KO knockout; MAP1LC3/LC3 microtubule associated protein 1 light chain 3; NRBF2 nuclear receptor binding factor 2; OPRD1/DOR opioid receptor delta 1 PIK3C3/VPS34 phosphatidylinositol 3-kinase catalytic subunit type 3; PIK3R4/VPS15 phosphoinositide-3-kinase regulatory subunit 4; PtdIns3K class III phosphatidylinositol 3-kinase; PtdIns3P phosphatidylinositol-3-phosphate; RUBCN rubicon autophagy regulator; SQSTM1/p62 sequestosome 1; UVRAG UV radiation resistance associated; VPS vacuolar protein sorting; WT wild type.

Keywords: ATG14; BECN2; PtdIns3K; autophagy; coiled-coil.

Abstract

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