Multi-omic analysis of the extension of broccoli quality during storage by folic acid

Yaqi Zhao; Junyan Shi; Bihong Feng; Shuzhi Yuan; Xiaozhen Yue; Wenlin Shi; Zhicheng Yan; Dongying Xu; Jinhua Zuo; Qing Wang

doi:10.1016/j.jare.2023.07.001

Multi-omic analysis of the extension of broccoli quality during storage by folic acid

J Adv Res. 2024 May:59:65-78. doi: 10.1016/j.jare.2023.07.001. Epub 2023 Jul 3.

Authors

Yaqi Zhao¹, Junyan Shi², Bihong Feng³, Shuzhi Yuan², Xiaozhen Yue², Wenlin Shi⁴, Zhicheng Yan², Dongying Xu², Jinhua Zuo⁵, Qing Wang⁶

Affiliations

¹ Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Institute of Agri-food Processing and Nutrition, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China; State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Agriculture, Guangxi University, Nanning 530004, China.
² Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Institute of Agri-food Processing and Nutrition, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
³ College of Agriculture, Guangxi University, Nanning 530004, China.
⁴ Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Institute of Agri-food Processing and Nutrition, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China; College of Agriculture, Guangxi University, Nanning 530004, China.
⁵ Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Institute of Agri-food Processing and Nutrition, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. Electronic address: zuojinhua@iapn.org.cn.
⁶ Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Institute of Agri-food Processing and Nutrition, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. Electronic address: wangqing@iapn.org.cn.

PMID: 37406731
DOI: 10.1016/j.jare.2023.07.001

Abstract

Introduction: Folic acid (FA) is a critical metabolite in all living organisms and an important nutritional component of broccoli. Few studies have been conducted on the impact of an exogenous application of FA on the postharvest physiology of fruits and vegetables during storage. In this regard, the mechanism by which an exogenous application of FA extends the postharvest quality of broccoli is unclear.

Objective: This study utilized a multicomponent analysis to investigate how an exogenous application of FA effects the postharvest quality of broccoli.

Methods: Broccoli was soaked in 5 mg/L FA for 10 min and the effect of the treatment on the appearance and nutritional quality of broccoli was evaluated. These data were combined with transcriptomic, metabolomic, and DNA methylation data to provide insight into the potential mechanism by which FA delays senescence.

Results: The FA treatment inhibited the yellowing of broccoli during storage. CHH methylation was identified as the main type of methylation that occurs in broccoli and the FA treatment was found to inhibit DNA methylation, promote the accumulation of endogenous FA and chlorophyl, and inhibit ethylene biosynthesis in stored broccoli. The FA treatment also prevented the formation of off-odors by inhibiting the degradation of glucosinolate.

Conclusions: FA treatment inhibited the loss of nutrients during the storage of broccoli, delayed its yellowing, and inhibited the generation of off-odors. Our study provides deeper insight into the mechanism by which the postharvest application of FA delays postharvest senescence in broccoli and provides the foundation for further studies of postharvest metabolism in broccoli.

Keywords: Broccoli; DNA methylation; Folic acid; Metabolomic; Transcriptomic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brassica* / metabolism
DNA Methylation*
Ethylenes / metabolism
Folic Acid* / metabolism
Food Storage* / methods
Gene Expression Regulation, Plant
Metabolomics / methods
Multiomics
Nutritive Value
Transcriptome

Substances

Folic Acid
Ethylenes