Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology

Maryam Alowaysi; Mohammad Al-Shehri; Moayad Baadhaim; Hajar AlZahrani; Doaa Aboalola; Mustafa Daghestani; Heba Hashem; Rawan Aljahdali; Rayan Salem; Adel Alharbi; Mohammed Muharraq; Khaled Alghamdi; Fawaz Alsobiy; Asima Zia; Robert Lehmann; Jesper Tegner; Khaled Alsayegh

doi:10.1016/j.scr.2023.103158

Generation of myoglobin (MB)-knockout human embryonic stem cell (hESC) line (KAIMRCe002-A-1S) using CRISPR/Cas9 technology

Stem Cell Res. 2023 Sep:71:103158. doi: 10.1016/j.scr.2023.103158. Epub 2023 Jun 25.

Authors

Maryam Alowaysi¹, Mohammad Al-Shehri¹, Moayad Baadhaim¹, Hajar AlZahrani¹, Doaa Aboalola¹, Mustafa Daghestani², Heba Hashem³, Rawan Aljahdali³, Rayan Salem³, Adel Alharbi⁴, Mohammed Muharraq⁴, Khaled Alghamdi⁴, Fawaz Alsobiy⁴, Asima Zia⁵, Robert Lehmann⁵, Jesper Tegner⁶, Khaled Alsayegh⁷

Affiliations

¹ King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia.
² King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia; Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia.
³ Department of Pathology and Laboratory Medicine, Ministry of the National Guard - Health Affairs, Jeddah, Saudi Arabia.
⁴ Forensic Laboratories, Criminal Evidence Department, Jeddah, Saudi Arabia.
⁵ Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
⁶ Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
⁷ King Abdullah International Medical Research Center (KAIMRC), King Saud bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Jeddah, Saudi Arabia. Electronic address: alsayeghkh@mngha.med.sa.

PMID: 37406498
DOI: 10.1016/j.scr.2023.103158

Abstract

Myoglobin (MB) is a cytoplasmic hemoprotein that is predominantly expressed in the heart and oxidative myofibers of skeletal muscle. It has been demonstrated that MB binds to oxygen and promotes its diffusion for energy production in the mitochondria. Recently, MB was found to be expressed in different forms of malignant tumors and cancer cell lines. Further studies using gene disruption technology will enhance the understanding of MB's role in human cardiovascular biology and cancers. Here, we describe the generation of a homozygous MB knockout in human embryonic stem cells (hESC-MB^-/-) via CRISPR/Cas9 to study MB function in human biology and diseases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Line
Human Embryonic Stem Cells* / metabolism
Humans
Myoglobin* / genetics
Myoglobin* / metabolism
Technology

Substances

Myoglobin