Effect of combined in ovo administration of zinc glycine chelate (Zn-Gly) and a multistrain probiotic on the modulation of cellular and humoral immune responses in broiler chickens

Artur Ciszewski; Łukasz Jarosz; Agnieszka Marek; Katarzyna Michalak; Zbigniew Grądzki; Beata Kaczmarek; Anna Rysiak

doi:10.1016/j.psj.2023.102823

Effect of combined in ovo administration of zinc glycine chelate (Zn-Gly) and a multistrain probiotic on the modulation of cellular and humoral immune responses in broiler chickens

Poult Sci. 2023 Sep;102(9):102823. doi: 10.1016/j.psj.2023.102823. Epub 2023 May 29.

Authors

Artur Ciszewski¹, Łukasz Jarosz¹, Agnieszka Marek², Katarzyna Michalak³, Zbigniew Grądzki¹, Beata Kaczmarek⁴, Anna Rysiak⁵

Affiliations

¹ Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-612, Poland.
² Department of Preventive Veterinary and Avian Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-950, Poland.
³ Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-612, Poland. Electronic address: zespollukasza@gmail.com.
⁴ Department and Clinic of Animal Internal Diseases, Sub-Department of Internal Diseases of Farm Animals and Horses, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-612, Poland.
⁵ Department of Botany, Mycology, and Ecology, Maria Curie-Skłodowska University, Lublin 20-033, Poland.

Abstract

The aim of the study was to determine the effect of in ovo administration of zinc glycine chelate (Zn-Gly), and a multistrain probiotic on the hatchability and selected parameters of the cellular and humoral immune response of chickens. The study was conducted on 1,400 fertilized eggs from commercial broiler breeders (Ross x Ross 708). Material for the study consisted of peripheral blood and spleens of chicks taken 12 h and 7 d after hatching. The results showed that both combined and single in ovo administration of the multistrain probiotic and zinc glycine chelate significantly reduced hatchability of chicks. The flow cytometry study showed that the highest percentage of CD4⁺ T cells, CD4⁺CD25⁺, and high expression of KUL01 in the serum were obtained in the group supplemented with probiotic and Zn-Gly both 12 h and 7 d after hatching. In birds supplemented with probiotic and zinc chelate, a high percentage of TCRγδ⁺ cells was found in serum and spleen 12 h after hatching and in serum after 7 d. The percentage of Bu-1A⁺ lymphocytes in serum and spleen 12 h and 7 d after hatching was the highest in the group supplemented with probiotic and Zn-Gly. The highest expression of CD79A was observed in the group supplemented only with zinc chelate. There were no significant differences in the percentage of CD4⁺ cells in the spleens of birds in the groups receiving the multistrain probiotic at 12 h after hatching, and after 7 d, the percentage of CD4⁺ T cells was lower in the experimental groups than in the control group. The percentage of CD8⁺ cells in the serum of birds after hatching was lower in the group supplemented with multistrain probiotic and Zn-Gly than in the control group, but reached the highest value on d 7 after hatching. The obtained results confirm the strong effect of the combined administration of a multistrain probiotic and Zn-Gly chelate on lymphocyte proliferation and stimulation of cellular immune mechanisms in birds.

Keywords: chicken; flow cytometry; in-ovo; multistrain probiotic; zinc glycine chelate.

MeSH terms

Animals
Chickens* / metabolism
Immunity, Humoral
Probiotics* / pharmacology
Zinc / metabolism

Substances

zinc glycinate
Zinc