Girdling promotes tomato fruit enlargement by enhancing fruit sink strength and triggering cytokinin accumulation

Abstract

Girdling is a horticultural technique that enhances fruit size by allocating more carbohydrates to fruits, yet its underlying mechanisms are not fully understood. In this study, girdling was applied to the main stems of tomato plants 14 days after anthesis. Following girdling, there was a significant increase in fruit volume, dry weight, and starch accumulation. Interestingly, although sucrose transport to the fruit increased, the fruit's sucrose concentration decreased. Girdling also led to an increase in the activities of enzymes involved in sucrose hydrolysis and AGPase, and to an upregulation in the expression of key genes related to sugar transport and utilization. Moreover, the assay of carboxyfluorescein (CF) signal in detached fruit indicated that girdled fruits exhibited a greater ability to take up carbohydrates. These results indicate that girdling improves sucrose unloading and sugar utilization in fruit, thereby enhancing fruit sink strength. In addition, girdling induced cytokinin (CK) accumulation, promoted cell division in the fruit, and upregulated the expression of genes related to CK synthesis and activation. Furthermore, the results of a sucrose injection experiment suggested that increased sucrose import induced CK accumulation in the fruit. This study sheds light on the mechanisms by which girdling promotes fruit enlargement and provides novel insights into the interaction between sugar import and CK accumulation.

Keywords: Solanum lycopersicum L.; carbohydrate allocation; sink strength; sucrose signal; sucrose unloading; trans-zeatin.