Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides

Yalpi Karthik; Manjula Ishwara Kalyani; Srinivasa Krishnappa; Krishnaveni Ramakrishna; Samy M Sayed; Ohud Muslat Aharthy; Seham Sater Alhelaify; Muntazir Mushtaq

doi:10.3389/fmicb.2023.1152985

Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides

Front Microbiol. 2023 Jun 16:14:1152985. doi: 10.3389/fmicb.2023.1152985. eCollection 2023.

Authors

Yalpi Karthik¹, Manjula Ishwara Kalyani¹, Srinivasa Krishnappa², Krishnaveni Ramakrishna³, Samy M Sayed⁴, Ohud Muslat Aharthy⁵, Seham Sater Alhelaify⁵, Muntazir Mushtaq⁶

Affiliations

¹ Department of Studies and Research in Microbiology, Mangalore University, Kodagu, Karnataka, India.
² Department of Studies and Research in Biochemistry, Mangalore University, Kodagu, Karnataka, India.
³ Department of Studies and Research in Microbiology, Vijayanagara Sri Krishnadevaraya University, Ballari, Karnataka, India.
⁴ Department of Science and Technology, University College-Ranyah, Taif University, Taif, Saudi Arabia.
⁵ Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia.
⁶ MS Swaminathan School of Agriculture, Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh, India.

Abstract

Streptomyces is a group of microbes known for antibiotic production and has contributed to more than 70% of present commercially available antibiotics. These antibiotics are important in the management, protection, and treatment of chronic illnesses. In the present study, the isolated S. tauricus strain from mangrove soil in Mangalore, India (GenBank accession number: MW785875) was subjected for differential cultural characterization, phenotype involving brown pigmentation, filamentous mycelia, and ash-colored spore production was observed using field emission scanning electron microscopy (FESEM) analysis revealing filamentous mycelia possessing a straight spore chain. Spores were visualized as elongated, rod-shaped, smooth surfaces with curved edges. After optimized growth conditions for S. tauricus on starch-casein agar medium, the GC/MS analysis of S. tauricus intracellular extract detected bioactive compounds reported for pharmacological applications. Analyzed using the NIST library, most of the bioactive compounds identified in intracellular extract had molecular weights of less than 1 kDa. On the PC3 cell line, the Sephadex G-10 partially purified eluted peak protein fraction demonstrated significant anticancer activity. The LCMS analysis revealed the presence of Tryprostatin B, Fumonisin B1, Microcystin LR, and Surfactin C with molecular weights below 1 kDa. This study found that small molecular weight microbial compounds are more effective in a variety of biological applications.

Keywords: FESEM; Streptomyces tauricus; anticancer; chromatography; mangroves.