MUC13 promotes the development of esophageal cancer by upregulating the expression of O-glycan process-related molecules

Yi Han; Gang Chen; Shiyu Liu; Guangqing Zhou; Xinxin Xu; Haihan Zhang; Zhentao Li; Chuannan Wu; Yulan Liu; Kai Fang; Guangxia Chen

doi:10.1007/s12672-023-00713-3

MUC13 promotes the development of esophageal cancer by upregulating the expression of O-glycan process-related molecules

Discov Oncol. 2023 Jul 3;14(1):123. doi: 10.1007/s12672-023-00713-3.

Authors

Yi Han^#¹, Gang Chen^#², Shiyu Liu¹, Guangqing Zhou³, Xinxin Xu³, Haihan Zhang¹, Zhentao Li¹, Chuannan Wu¹, Yulan Liu⁴, Kai Fang⁴, Guangxia Chen^#⁵

Affiliations

¹ Department of Gastroenterology, The First People's Hospital of Xuzhou, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, 221002, China.
² Department of Plastic Surgery, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
³ Xuzhou Medical University, Xuzhou, 221002, China.
⁴ The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, 610051, China.
⁵ Department of Gastroenterology, The First People's Hospital of Xuzhou, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou, 221002, China. gx_chen2010@163.com.

^# Contributed equally.

Abstract

Background: Esophageal cancer is one of the most common malignant tumors in the world, which is characterized by poor prognosis, aggressiveness, and poor survival. Mucin 13 (MUC13) is a member of the membrane-bound mucin and located on chromosome 3q21.2 and consists of α and β subunits. It has been found that MUC13 is overexpressed in a variety of tumor cells and acts a vital role in the invasiveness and malignant progression of several types of tumors. However, the role and regulatory mechanism of MUC13 in the progression of esophageal cancer remain unclear.

Methods: The expression level of MUC13 was detected in 15 esophageal cancer tissues and 15 pairs of adjacent nontumor tissues by immunohistochemistry (IHC). In addition, the expression of MUC13 mRNA level in human esophageal cancer cell lines (EC9706 and ECA109 and TE-1) was measured by qRT-PCR. In vitro, after silencing MUC13 with lentiviral interference technology, CCK8 assay, clone formation assay, and flow cytometry were applied to investigate the proliferation activity, clone formation ability and anti-apoptosis ability of EC9706 and ECA109 cells. The tumor xenograft growth assay was used to confirm the influence of MUC13 knockdown on the growth of esophageal tumors in vivo. The qRT-PCR assay and western blot experiments were taken to study the mechanism of MUC13 regulating the proproliferation and antiapoptotic of esophageal cancer.

Results: The results showed that MUC13 was overexpressed in esophageal cancer tissues and cell lines (EC9706 and ECA109 and TE-1), especially in EC9706 and ECA109 cells, but low expressed in human esophageal epithelial cell line (HEEC). Next, silencing MUC13 inhibits proliferation, blocks cell cycle progression, and promotes cell apoptosis in vitro, and restrains the growth of esophageal cancer tissues in vivo. Finally, MUC13 affects the proproliferation and antiapoptotic by regulating the expression of GLANT14, MUC3A, MUC1, MUC12, and MUC4 that closely related to O-glycan process.

Conclusions: This study proved that MUC13 is an important molecule that regulates the O-glycan process and then affects the progress of esophageal cancer. MUC13 may be a novel therapeutic target for patients with esophageal cancer.

Keywords: Apoptosis; Esophageal cancer; MUC13; Proliferation.

Abstract

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