Metallochaperone protein OsHIPP17 regulates the absorption and translocation of cadmium in rice (Oryza sativa L.)

Yang Shi; Wenjun Jiang; Mingyu Li; Nan Jiang; Yanyan Huang; Mengting Wang; Zhiye Du; Ji Chen; Jiahao Li; Longying Wu; Min Zhong; Ju Yang; Jin Huang

doi:10.1016/j.ijbiomac.2023.125607

Metallochaperone protein OsHIPP17 regulates the absorption and translocation of cadmium in rice (Oryza sativa L.)

Int J Biol Macromol. 2023 Aug 1:245:125607. doi: 10.1016/j.ijbiomac.2023.125607. Epub 2023 Jun 28.

Authors

Yang Shi¹, Wenjun Jiang¹, Mingyu Li¹, Nan Jiang¹, Yanyan Huang², Mengting Wang¹, Zhiye Du¹, Ji Chen³, Jiahao Li¹, Longying Wu¹, Min Zhong¹, Ju Yang¹, Jin Huang⁴

Affiliations

¹ College of Ecology and Environment, Chengdu University of Technology, Sichuan 610059, China.
² State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Sichuan 611130, China.
³ College of Agronomy, Sichuan Agricultural University, Sichuan 611130, China.
⁴ College of Ecology and Environment, Chengdu University of Technology, Sichuan 610059, China. Electronic address: huangjin18@cdut.edu.cn.

PMID: 37390996
DOI: 10.1016/j.ijbiomac.2023.125607

Abstract

Heavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in regulating heavy metal responding activities in plants. Yet only a handful of studies have characterized the functions of HIPPs. In this study, a novel HIPP member OsHIPP17 was functionally characterized, which was involved in the tolerance of yeast and plants to cadmium (Cd). The Cd accumulation in yeast cells was increased due to the overexpression of OsHIPP17. Nevertheless, the overexpression of OsHIPP17 in Arabidopsis thaliana resulted in compromised growth under Cd stress. Meanwhile, the mutation of OsHIPP17 resulted in 38.9-40.9 % increase of Cd concentration in rice roots as well as 14.3-20.0 % decrease of Cd translocation factor. Further investigation of the genes responsible for Cd absorption and transporter indicated that the expression levels of these genes were also perturbed. In addition, two OsHIPP17-interacting proteins, OsHIPP24 and OsLOL3 were identified in a yeast two hybrid assay. Further analysis of their functions revealed that OsHIPP24 or OsLOL3 may be involved in the regulation of Cd tolerance by OsHIPP17 in rice. All above results implied that OsHIPP17 may affect Cd resistance by regulating the absorption and translocation of Cd in rice.

Keywords: Absorption; Cadmium; HIPP; Rice; Transportation.