T4PPVB-COP composite-driven innovative electrochemiluminescence aptasensor for ultra-sensitive detection of chlorpyrifos

Yanping Li; Qiong Hu; Jihui Zhang; Hong Zhou; Na Wang; Yishan Fang; Bo Cui

doi:10.1016/j.foodchem.2023.136713

T4PPVB-COP composite-driven innovative electrochemiluminescence aptasensor for ultra-sensitive detection of chlorpyrifos

Food Chem. 2023 Nov 30:427:136713. doi: 10.1016/j.foodchem.2023.136713. Epub 2023 Jun 25.

Authors

Yanping Li¹, Qiong Hu², Jihui Zhang¹, Hong Zhou¹, Na Wang¹, Yishan Fang³, Bo Cui¹

Affiliations

¹ School of Food Science and Engineering, State Key Laboratory of Biobased Material and Green Papermaking, School of Materials Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
² Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, College of Chemistry and Food Science, Yulin Normal University, Yulin 537000, Guangxi, China.
³ School of Food Science and Engineering, State Key Laboratory of Biobased Material and Green Papermaking, School of Materials Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China. Electronic address: fangysh123@qlu.edu.cn.

PMID: 37390738
DOI: 10.1016/j.foodchem.2023.136713

Abstract

Herein, an enhanced electrochemiluminescence (ECL) aptasensor driven by a complex (T4PPVB-COP@CdS QDs) with large specific surface area and high stability was constructed for highly sensitive detection of chlorpyrifos (CPF), using electrostatic interactions and signal amplification techniques. In the presence of CPF, the specific binding between the aptamer and CPF caused partial detachment of the aptamer from the sensor, thus restoring the ECL signal. Notably, gold nanoparticles functionalized with streptavidin (SA) as signal enhancers further amplified the ECL signal in specific interactions with aptamers, thereby improving the sensitivity of the assay. Based on this, the proposed ECL aptasensor demonstrated significant detection performance for CPF with a linear range of 1-10⁷ pg/mL and a LOD of 0.34 pg/mL. Furthermore, the feasibility of the ECL aptasensor was validated by the detection and analysis of CPF in real samples, which also provided a broad reference value for bioanalysis.

Keywords: Aptasensor; CdS QDs; Chlorpyrifos; Electrochemiluminescence; Signal amplification; T4PPVB-COP.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques* / methods
Chlorpyrifos*
Electrochemical Techniques / methods
Gold
Limit of Detection
Luminescent Measurements / methods
Metal Nanoparticles*

Substances

Chlorpyrifos
Gold
Aptamers, Nucleotide