Photoelectrochemical biosensor based on SiW12@CdS quantum dots for the highly sensitive detection of HPV 16 DNA

Yao Cheng; Chaoyue Sun; Yuhua Chang; Jiayin Wu; Zhihao Zhang; Yunqing Liu; Shenguang Ge; Zhao Li; Xiao Li; Liang Sun; Dejin Zang

doi:10.3389/fbioe.2023.1193052

Photoelectrochemical biosensor based on SiW₁₂@CdS quantum dots for the highly sensitive detection of HPV 16 DNA

Front Bioeng Biotechnol. 2023 Jun 14:11:1193052. doi: 10.3389/fbioe.2023.1193052. eCollection 2023.

Authors

Yao Cheng¹, Chaoyue Sun^{1

2}, Yuhua Chang³, Jiayin Wu¹, Zhihao Zhang¹, Yunqing Liu², Shenguang Ge², Zhao Li⁴, Xiao Li^{5

6}, Liang Sun¹, Dejin Zang¹

Affiliations

¹ National Key Laboratory of Advanced Drug Delivery and Release System, NHC Key Laboratory of Biotechnology Drugs (Shandong Academy of Medical Sciences), Key Lab for Rare and Uncommon Diseases of Shandong Province, School of Pharmacy and Pharmaceutical Sciences, Institute of Materia Medica, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.
² Institute for Advanced Interdisciplinary Research (iAIR), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, China.
³ Shandong Provincial Maternal and Child Healthcare Hospital, Jinan, China.
⁴ Suzhou KunTao Intelligent Manufacturing Technology Co., Ltd., Suzhou, China.
⁵ NMPA Key Laboratory for Quality Evaluation of Medical Materials and Biological Protective Devices, Jinan, China.
⁶ Shandong Institute of Medical Device and Pharmaceutical Packaging Inspection, Jinan, China.

Abstract

A highly sensitive biosensor for detecting HPV 16 DNA was prepared based on Keggin-type polyoxometalate (SiW₁₂)-grafted CdS quantum dots (SiW₁₂@CdS QDs) and colloidal gold nanoparticles (Au NPs), which exhibited remarkable selectivity and sensitivity upon target DNA detection because of its excellent photoelectrochemical (PEC) response. Here, an enhanced photoelectronic response ability was achieved with the strong association of SiW₁₂@CdS QDs by polyoxometalate modification, which was developed through a convenient hydrothermal process. Furthermore, on Au NP-modified indium tin oxide slides, a multiple-site tripodal DNA walker sensing platform coupled with T7 exonuclease was successfully fabricated with SiW₁₂@CdS QDs/NP DNA as a probe for detecting HPV 16 DNA. Due to the remarkable conductivity of Au NPs, the photosensitivity of the as-prepared biosensor was improved in an $I_{3}^{-} / I^{-}$ solution and avoided the use of other regents toxic to living organisms. Finally, under optimized conditions, the as-prepared biosensor protocol demonstrated wide linear ranges (15-130 nM), with a limit of detection of 0.8 nM and high selectivity, stability, and reproducibility. Moreover, the proposed PEC biosensor platform offers a reliable pathway for detecting other biological molecules with nano-functional materials.

Keywords: HPV 16 DNA; biosensor; photoelectrochemistry; polyoxometalates; quantum dots.

Grants and funding

This work was supported by the National Natural Science Foundation of China (No. 21801153, 31970636); International (regional) cooperative research and exchange programs (No. 32211530422); Shandong Institute of Medical device and pharmaceutical packaging inspection program (NB202203); Academic promotion program of Shandong First Medical University (No. 2019LJ003) and Wujiang Development Zone Chongben Technology Leading Talent Plan.