Decreased acetylation of HDGF improves oviduct production in Rana dybowskii, Rana amurensis, and Rana huanrenensis

Da Liu; Qirong Li; Tianjia Liu; Yi Zhang; Ran Zheng; Huimin Liu; Zhijing Yang; Qi Yu; Chao Lin; Zhidong Qiu; Dongxu Wang; Yiping Li

doi:10.1016/j.cbd.2023.101102

Decreased acetylation of HDGF improves oviduct production in Rana dybowskii, Rana amurensis, and Rana huanrenensis

Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep:47:101102. doi: 10.1016/j.cbd.2023.101102. Epub 2023 Jun 13.

Authors

Da Liu¹, Qirong Li², Tianjia Liu³, Yi Zhang¹, Ran Zheng¹, Huimin Liu⁴, Zhijing Yang⁴, Qi Yu¹, Chao Lin⁵, Zhidong Qiu⁶, Dongxu Wang⁷, Yiping Li⁸

Affiliations

¹ Department of Pharmacy, Changchun University of Chinese Medicine, Changchun, China.
² Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China.
³ The Third Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China.
⁴ Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China.
⁵ School of grain science and technology, Jilin Business and Technology College, Changchun, China.
⁶ Department of Pharmacy, Changchun University of Chinese Medicine, Changchun, China. Electronic address: qzdcczy@163.com.
⁷ Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China. Electronic address: wang_dong_xu@jlu.edu.cn.
⁸ Department of Pharmacy, Changchun University of Chinese Medicine, Changchun, China. Electronic address: czdliyiping@163.com.

PMID: 37384958
DOI: 10.1016/j.cbd.2023.101102

Abstract

The oviduct of female Rana dybowskii is a functional food and can be used as a component of Traditional Chinese medicine. The differentially expressed genes enriched was screened in cell growth of three Rana species. We quantitatively analyzed 4549 proteins using proteomic techniques, enriching the differentially expressed proteins of Rana for growth and signal transduction. The results showed that log2 expression of hepatoma-derived growth factor (HDGF) was increased. We further verified 5 specific differential genes (EIF4a, EIF4g, HDGF1, HDGF2 and SF1) and found that HDGF expression was increased in Rana dybowskii. Through acetylation modification analysis, we identified 1534 acetylation modification sites in 603 proteins, including HDGF, and found that HDGF acetylation expression was significantly reduced in Rana dybowskii. Our results suggest that HDGF is involved in the development of oviductus ranae, which is regulated by acetylation modification.

Keywords: Acetylation modifications; HDGF; Oviductus; Proteomics; Rana.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylation
Animals
Female
Humans
Oviducts* / metabolism
Proteomics*
Ranidae / metabolism

Substances

hepatoma-derived growth factor