Simultaneous determination of residues of multiple pesticides and their metabolites in citrus and orange juice from markets in China: residue levels and dietary risk assessment

Abstract

Consumers are becoming more concerned about pesticide residues in food. Since citrus represent a significant portion of the diet, it is appropriate to monitor the pesticide residues in citrus. In this paper, we modified a QuEChERS method combined with HPLC-MS/MS to investigate residue levels of 15 pesticides and 3 metabolites in citrus (whole fruit and pulp) and orange juice from the markets in China. And the dietary exposure risks were evaluated by using the hazard quotient (HQ) and hazard index (HI) methods based on deterministic and probabilistic models. The recoveries of the modified method ranged from 70 to 112% at three spike levels of 0.005-0.5 mg/kg with relative standard deviations of 1.0-18.1%. Pesticide residues were detected in 85.84% of the whole citrus and 40.00% of pulp, with concentrations ranging from 0.005 to 0.47 mg/kg, which did not exceed their maximum residue limits (MRLs) in China. The HQ (0.01-11.41%) and HI (0.07-16.2%) were both less than 100%, demonstrating that chronic, acute, and cumulative dietary risks were acceptable. Notably, the risk for children (1-6 years old, 1.96-16.2%) was higher than that for the general population (0.76-6.25%). The results of our study can provide a valuable reference for regular monitoring to protect public health and ensure pesticide management.

Keywords: Citrus; Deterministic and probabilistic models; Dietary risk assessment; Metabolites; Pesticide residues.