Effect of tumor CD276 expression on infiltrating immune cells and clinicopathological features of prostate cancer

Ji Liu; Aimaitiaji Kadier; Yadong Guo; Wentao Zhang; Haotian Chen; Zhijin Zhang; Changcheng Guo; Yue Zhang; Meiyu Bao; Jiang Geng; Junfeng Zhang; Shiyu Mao; Xudong Yao

doi:10.1038/s41391-023-00690-2

Effect of tumor CD276 expression on infiltrating immune cells and clinicopathological features of prostate cancer

Prostate Cancer Prostatic Dis. 2023 Jun 28. doi: 10.1038/s41391-023-00690-2. Online ahead of print.

Authors

Ji Liu^#^{1

2}, Aimaitiaji Kadier^#^{1

2}, Yadong Guo^#^{1

2}, Wentao Zhang^{1

2}, Haotian Chen^{1

2}, Zhijin Zhang^{1

2}, Changcheng Guo^{1

2}, Yue Zhang³, Meiyu Bao³, Jiang Geng^{1

2}, Junfeng Zhang^{4

5}, Shiyu Mao^{6

7}, Xudong Yao^{8

9}

Affiliations

¹ Department of Urology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.
² Urologic Cancer Institute, School of Medicine, Tongji University, Shanghai, China.
³ Department of Central Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People's Hospital, Shanghai, 200435, China.
⁴ Department of Urology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China. zhangjunfeng@alumni.tongji.edu.cn.
⁵ Urologic Cancer Institute, School of Medicine, Tongji University, Shanghai, China. zhangjunfeng@alumni.tongji.edu.cn.
⁶ Department of Urology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China. maoshiyu1144@sina.com.
⁷ Urologic Cancer Institute, School of Medicine, Tongji University, Shanghai, China. maoshiyu1144@sina.com.
⁸ Department of Urology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China. yaoxudong1967@163.com.
⁹ Urologic Cancer Institute, School of Medicine, Tongji University, Shanghai, China. yaoxudong1967@163.com.

^# Contributed equally.

PMID: 37380802
DOI: 10.1038/s41391-023-00690-2

Abstract

Background: Advanced prostate cancer (PCa) is often resistant to immunotherapy. In this study, we examined the role of CD276 in mediating immunotherapeutic effects through changes in immune cell infiltration.

Methods: Using transcriptomic and proteomic analyses, CD276 was identified as a potential target for immunotherapy. Subsequent in vivo and in vitro experiments confirmed its role as a potential mediator of immunotherapeutic effects.

Results: Multi-omic analysis suggested that CD276 was identified as a key molecule regulating the immune microenvironment (IM). In vivo experiments revealed that CD276 knockdown was found to enhance CD8⁺ T cell infiltration into the IM. Immunohistochemical analysis of PCa samples further confirmed the same findings.

Conclusion: CD276 was found to inhibit the enrichment of CD8+ T cells in PCa. Thus, CD276 inhibitors may be potential targets for immunotherapy.