Head or tail? A molecular dynamics approach to the complex structure of TNF-associated factor TRAF2

Biomol Concepts. 2023 Jun 28;14(1). doi: 10.1515/bmc-2022-0031. eCollection 2023 Jan 1.

Abstract

Tumor necrosis factor receptor-associated factor proteins (TRAFs) are trimeric proteins that play a fundamental role in signaling, acting as intermediaries between the tumor necrosis factor (TNF) receptors and the proteins that transmit the downstream signal. The monomeric subunits of all the TRAF family members share a common tridimensional structure: a C-terminal globular domain and a long coiled-coil tail characterizing the N-terminal section. In this study, the dependence of the TRAF2 dynamics on the length of its tail was analyzed in silico. In particular, we used the available crystallographic structure of a C-terminal fragment of TRAF2 (168 out of 501 a.a.), TRAF2-C, and that of a longer construct, addressed as TRAF2-plus, that we have re-constructed using the AlphaFold2 code. The results indicate that the longer N-terminal tail of TRAF2-plus has a strong influence on the dynamics of the globular regions in the protein C-terminal head. In fact, the quaternary interactions among the TRAF2-C subunits change asymmetrically in time, while the movements of TRAF2-plus monomers are rather limited and more ordered than those of the shorter construct. Such findings shed a new light on the dynamics of TRAF subunits and on the protein mechanism in vivo, since TRAF monomer-trimer equilibrium is crucial for several reasons (receptor recognition, membrane binding, hetero-oligomerization).

Keywords: TRAFs; molecular dynamics; protein contact networks; quaternary interaction.

MeSH terms

  • Molecular Dynamics Simulation*
  • NF-kappa B / metabolism
  • Protein Binding
  • Receptors, Tumor Necrosis Factor* / chemistry
  • Receptors, Tumor Necrosis Factor* / metabolism
  • Signal Transduction
  • TNF Receptor-Associated Factor 2 / chemistry
  • TNF Receptor-Associated Factor 2 / metabolism
  • Ubiquitin-Protein Ligases

Substances

  • TNF Receptor-Associated Factor 2
  • Receptors, Tumor Necrosis Factor
  • Ubiquitin-Protein Ligases
  • NF-kappa B