Genome-Wide Characterization and Sequence Polymorphism Analyses of Glycine max Fibrillin (FBN) Revealed Its Role in Response to Drought Condition

Muhammad Zeshan Zafer; Muhammad Hammad Nadeem Tahir; Zulqurnain Khan; Muhammad Sajjad; Xiangkuo Gao; Muhammad Amir Bakhtavar; Ummara Waheed; Maria Siddique; Zhide Geng; Shoaib Ur Rehman

doi:10.3390/genes14061188

Genome-Wide Characterization and Sequence Polymorphism Analyses of Glycine max Fibrillin (FBN) Revealed Its Role in Response to Drought Condition

Genes (Basel). 2023 May 29;14(6):1188. doi: 10.3390/genes14061188.

Authors

Affiliations

¹ SINO-PAK Joint Research Laboratory, Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, Pakistan.
² Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Shareef, University of Agriculture, Multan 66000, Pakistan.
³ Department of Biosciences, COMSATS University, Islamabad (CUI), Park Road, Islamabad 45550, Pakistan.
⁴ Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650204, China.
⁵ Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, Pakistan.

Abstract

The fibrillin (FBN) gene family is widely distributed in all photosynthetic organisms. Members of this gene family are involved in plant growth and development and their response to various biotic and abiotic stress factors. In this study, 16 members of FBN were identified in Glycine max and characterized by using different bioinformatics tools. Phylogenetic analysis classified FBN genes into seven groups. The presence of stress-related cis-elements in the upstream region of GmFBN highlighted their role in tolerance against abiotic stresses. To further decipher the function, physiochemical properties, conserved motifs, chromosomal localization, subcellular localization, and cis-acting regulatory elements were also analyzed. Gene expression analysis based on FPKM values revealed that GmFBNs greatly enhanced soybean drought tolerance and controlled the expression of several genes involved in drought response, except for GmFBN-4, GmFBN-5, GmFBN-6, GmFBN-7 and GmFBN-9. For high throughput genotyping, an SNP-based CAPS marker was also developed for the GmFBN-15 gene. The CAPS marker differentiated soybean genotypes based on the presence of either the GmFBN-15-G or GmFBN-15-A alleles in the CDS region. Association analysis showed that G. max accessions containing the GmFBN-15-A allele at the respective locus showed higher thousand seed weight compared to accessions containing the GmFBN-15-G allele. This research has provided the basic information to further decipher the function of FBN in soybean.

Keywords: CAPS; Glycine max; drought; fibrillin (FBN); genome-wide identification; marker-assisted breeding.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Droughts*
Fibrillins / genetics
Glycine max* / genetics
Glycine max* / metabolism
Phylogeny
Regulatory Sequences, Nucleic Acid

Substances

Fibrillins

Grants and funding

This research was funded by the Key Technology and Science Promotion Cooperation Based on the Whole Industrial Chain of Bean, Project Number: GHJD-2020025, and the Punjab Agricultural Research Board, Project Number: PARB-830.