Flow Cytometry Identification of Cell Compartments in the Murine Brain

Joel J D Moffet; Zachery Moore; Shannon J Oliver; Tahnee Towers; Misty R Jenkins; Saskia Freytag; James R Whittle; Sarah A Best

doi:10.1007/978-1-0716-3331-1_14

Flow Cytometry Identification of Cell Compartments in the Murine Brain

Methods Mol Biol. 2023:2691:185-198. doi: 10.1007/978-1-0716-3331-1_14.

Authors

Joel J D Moffet¹, Zachery Moore^{1

2}, Shannon J Oliver¹, Tahnee Towers¹, Misty R Jenkins^{2

3}, Saskia Freytag^{1

2}, James R Whittle^{1

2

4

5}, Sarah A Best^{6

7}

Affiliations

¹ Personalised Oncology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
² Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia.
³ Immunology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
⁴ Department of Medical Oncology, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
⁵ Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC, Australia.
⁶ Personalised Oncology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. best@wehi.edu.au.
⁷ Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia. best@wehi.edu.au.

PMID: 37355546
DOI: 10.1007/978-1-0716-3331-1_14

Abstract

Glioma can be modelled in the murine brain through the induction of genetically engineered mouse models or intracranial transplantation. Gliomas (oligodendroglioma and astrocytoma) are thought to arise from neuronal and glial progenitor populations in the brain and are poorly infiltrated by immune cells. An improved understanding of oligodendrocytes, astrocytes, and the immune environment throughout tumor development will enhance the analysis and development of brain cancer models. Here, we describe the isolation and analysis of murine brain cell types using a combination of flow cytometry and quantitative RT-PCR strategies to analyze these individual cell populations in vivo.

Keywords: Brain; Flow cytometry; Glial cells; Quantitative RT-PCR; Tissue dissociation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astrocytoma* / metabolism
Astrocytoma* / pathology
Brain / metabolism
Brain Neoplasms* / pathology
Flow Cytometry
Glioma* / pathology
Mice
Oligodendroglioma* / metabolism
Oligodendroglioma* / pathology