Multiplexed detection of biomarkers using a microfluidic chip integrated with mass-producible micropillar array electrodes

Chaozhan Chen; Bin Ran; Bo Liu; Xiaoxuan Liu; Ziteng Zhang; Yan Li; Hongchun Li; Minbo Lan; Yonggang Zhu

doi:10.1016/j.aca.2023.341450

Multiplexed detection of biomarkers using a microfluidic chip integrated with mass-producible micropillar array electrodes

Anal Chim Acta. 2023 Sep 1:1272:341450. doi: 10.1016/j.aca.2023.341450. Epub 2023 Jun 2.

Authors

Chaozhan Chen¹, Bin Ran¹, Bo Liu¹, Xiaoxuan Liu², Ziteng Zhang³, Yan Li³, Hongchun Li³, Minbo Lan⁴, Yonggang Zhu⁵

Affiliations

¹ School of Science, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China; School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China; Center for Microflows and Nanoflows, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China.
² School of Science, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China; Center for Microflows and Nanoflows, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China.
³ Shenzhen Third People's Hospital, Shenzhen, 518112, PR China.
⁴ Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
⁵ School of Science, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China; School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China; Center for Microflows and Nanoflows, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, PR China. Electronic address: zhuyonggang@hit.edu.cn.

PMID: 37355325
DOI: 10.1016/j.aca.2023.341450

Abstract

Quantifying multiple biomarkers with high sensitivity in tiny biological samples is essential to meet the growing demand for point-of-care testing. This paper reports the development of a novel microfluidic device integrated with mass-producible micropillar array electrodes (μAEs) for multiple biomarker detections. The μAE are mass-fabricated by soft lithography and hot embossing technique. Pt-Pd bimetallic nanoclusters (BNC) are modified on the surface of μAEs by constant potential (CP)/multi-potential step (MPS) electrodeposition strategies to improve the electroanalytical performance. The experimental result displays that Pt-Pd BNC/μAEs have good sensitivity enhancement compared with bare planar electrodes and bare μAEs, the enhancement being 56.5 and 9.5 times respectively, from the results of the H₂O₂ detection. Furthermore, glucose, uric acid and sarcosine were used as model biomarkers to show the biosensing capability with high sensitivity. The linear range and LOD of the glucose, uric acid and sarcosine detection are 0.1 mM-12 mM, 10 μM-800 μM and 2.5 μM-100 μM, 58.5, 3.4 and 0.4 μM, respectively. In particular, biosensing chips show wide linear ranges covering required detection ranges of glucose, uric acid and sarcosine in human serum, indicating the developed device has great potential in self-health management and clinical requirements.

Keywords: Biomarker detection; Electrochemical detection; High sensitivity; Mass-producible micropillar array electrode.

MeSH terms

Biomarkers
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Electrodes
Glucose
Humans
Hydrogen Peroxide
Microfluidics*
Sarcosine
Uric Acid

Substances

Uric Acid
Hydrogen Peroxide
Sarcosine
Glucose
Biomarkers