A dual-photoelectrode fuel cell-driven self-powered aptasensor based on the 1D/2D In2S3/MoS2@Fe-CNTs heterojunction for the ultrasensitive detection of Staphylococcus aureus

Abstract

A novel dual-electrode photo-fuel cell (PFC)-driven self-powered aptasensor was manufactured for the sensitive and selective detection of Staphylococcus aureus (S. aureus) using the one-dimensional (1D)/2D Schottky heterojunction comprising bimetallic indium/molybdenum sulfide nanosheets and iron-doped carbon nanotube (Fe-CNT) (denoted as In₂S₃/MoS₂@Fe-CNTs) as the photocathode. Given the generation of a robust interface at In₂S₃/MoS₂ and Fe-CNTs, the charge separation and transfer ability of photoexcited electron-hole pairs were enforced, thus improving the output voltage of the assembled PFC. In addition, the numerous active sites of the 1D/2D In₂S₃/MoS₂@Fe-CNTs Schottky heterojunction enabled the immobilization of large amounts of aptamer. Accordingly, the proposed PFC-driven self-powered aptasensor exhibited a wide linear range in 10-1 × 10⁷ CFU mL^-1 with a detection limit of 1.2 CFU mL^-1 toward S. aureus. High selectivity, excellent reproducibility, good stability, and acceptable regenerability, as well as great potential practicality, were also achieved for the detection of S. aureus using the developed PFC-driven self-powered aptasensor. This work not only provides a new photoactive material based on a robust 1D/2D Schottky heterojunction, but also constructs a novel PFC-based self-powered aptasensing strategy based on dual-photoelectrodes and with satisfactory performance for the detection of foodborne pathogens in diverse environments.

Keywords: 1D/2D Schottky heterojunction; Detection of Staphylococcus aureus; Dual-photoelectrode self-powered aptasensor; In(2)S(3)/MoS(2) nanosheets; Photo-fuel cell.