Comparative transcriptome analysis of gonads in male and female Pseudobagrus ussuriensis (Bagridae, Siluriformes)

Long Wang; Tianyu Guan; Jieyi Gu; Chuankun Zhu; Zhengjun Pan; Hui Wang; Jiale Li

doi:10.1016/j.cbd.2023.101105

Comparative transcriptome analysis of gonads in male and female Pseudobagrus ussuriensis (Bagridae, Siluriformes)

Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep:47:101105. doi: 10.1016/j.cbd.2023.101105. Epub 2023 Jun 19.

Authors

Long Wang¹, Tianyu Guan¹, Jieyi Gu², Chuankun Zhu², Zhengjun Pan², Hui Wang³, Jiale Li⁴

Affiliations

¹ Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huai'yin Normal University, Huai'an 223300, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
² Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huai'yin Normal University, Huai'an 223300, China.
³ Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huai'yin Normal University, Huai'an 223300, China. Electronic address: hytcwhh@hytc.edu.cn.
⁴ National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.

PMID: 37354751
DOI: 10.1016/j.cbd.2023.101105

Abstract

As an important aquaculture fish in the Heilongjiang River Basin, Pseudobagrus ussuriensis has high economic value, and all-male culture is beneficial to the economic development of this fish. In this study, the transcriptomes of gonads in males and females were analyzed, and some genes related to gonad development were found. A total of 82,931 unigenes were found (average length 1504 bp, N50 1829 bp). In addition, 4689 differentially expressed genes (DEGs; including 1424 genes upregulated and 3265 genes downregulated in males) were identified. Some genes associated with testis development (such as Dmrt1 and Ropn1l) were significantly upregulated in males, while genes related to ovary development (such as Wnt2, PLC, Cyp19a, ZP3) were significantly downregulated in males, demonstrating that these genes have a crucial influence on gonad development in P. ussuriensis. Some signaling pathways related to gonad development were found, such as the Wnt pathway and oocyte meiosis. The results of RNA-seq obtained in this study provide theoretical data for elucidating the potential mechanism of gonad development of P. ussuriensis and reliable genomic data for the establishment of mono-sex breeding of P. ussuriensis.

Keywords: Gonad development; Pseudobagrus ussuriensis; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Catfishes* / genetics
Female
Gene Expression Profiling
Gonads / metabolism
Male
RNA-Seq
Transcriptome*