Waterlogging stress alters the structure of sugar beet rhizosphere microbial community structure and recruiting potentially beneficial bacterial

Tai Li; Meihui Wang; Rufei Cui; Bingchen Li; Tong Wu; Yonglong Liu; Gui Geng; Yao Xu; Yuguang Wang

doi:10.1016/j.ecoenv.2023.115172

Waterlogging stress alters the structure of sugar beet rhizosphere microbial community structure and recruiting potentially beneficial bacterial

Ecotoxicol Environ Saf. 2023 Jun 21:262:115172. doi: 10.1016/j.ecoenv.2023.115172. Online ahead of print.

Authors

Tai Li¹, Meihui Wang¹, Rufei Cui¹, Bingchen Li¹, Tong Wu², Yonglong Liu¹, Gui Geng³, Yao Xu¹, Yuguang Wang⁴

Affiliations

¹ Heilongjiang Sugar Beet Engineering Technology Research Center, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74Xuefu Road, Harbin 150080, China; Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, 74Xuefu Road, Harbin 150080, China.
² College of Earth and Environmental Sciences, the University of Queensland, Brisbane, Queensland 4072, Australia.
³ Heilongjiang Sugar Beet Engineering Technology Research Center, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74Xuefu Road, Harbin 150080, China; Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, 74Xuefu Road, Harbin 150080, China; National Sugar Crop Improvement Centre, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74Xuefu Road, Harbin 150080, China. Electronic address: genggui01@163.com.
⁴ Heilongjiang Sugar Beet Engineering Technology Research Center, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74Xuefu Road, Harbin 150080, China; Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, 74Xuefu Road, Harbin 150080, China; National Sugar Crop Improvement Centre, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74Xuefu Road, Harbin 150080, China. Electronic address: wangyuguang@hlju.edu.cn.

PMID: 37354564
DOI: 10.1016/j.ecoenv.2023.115172

Abstract

Waterlogging has been shown to have a significant inhibitory effect on plant growth. However, the response mechanisms of the soil environment of sugar beet seedlings under waterlogging conditions still need to be fully understood. This study aimed to investigate the effects of waterlogging treatments on the content of effective nutrients and the microbial communities in the rhizosphere and non-rhizosphere using high-throughput sequencing. We set up waterlogging and non-waterlogging treatments, sampled sugar beet seedlings after 10 days of waterlogging, determined the effective soil nutrients in the rhizosphere and non-rhizosphere of the plants, and analyzed the differences in microbial diversity at ten days of waterlogging. The results showed that waterlogging significantly affected available potassium (AK) content. The Ak content of waterlogged soil was significantly higher than that of non-waterlogged soil. Waterlogging caused no significant difference in available nitrogen (AN) content and pH. Moreover, the plant growth-promoting bacteria Pseudomonas was significantly enriched in sugar beet waterlogged rhizospheres compared with the non-waterlogged ones. Similarly, the harmful fungi Gibellulopsis and Alternaria were enriched in sugar beet non-waterlogged rhizosphere. The network analysis revealed that waterlogging built a less complex root-microbial network than non-waterlogging. These findings implied that sugar beets subjected to waterlogging stress were enriched with beneficial microorganisms in the rhizosphere, potentially alleviating the stress.

Keywords: Beneficial bacteria; High throughput sequencing; Rhizosphere; Sugar beet; Waterlogging stress.