Enhancing sulfate reduction and hydrogen sulfide removal through gas stripping in the acidogenesis phase of a two-phase anaerobic process

Abstract

This study aims at evaluating two-phase and single-phase reactors for treating sulfate wastewater with low COD/SO₄^2- ratios. Additionally, a new process of gas stripping in an acidogenesis phase is proposed to reduce hydrogen sulfide (H₂S) inhibition and enhance biomethanation. The two-phase performed better than the single-phase in terms of COD removal, CH₄ production and H₂S resistance. After 30 days of stripping, the COD and sulfate degradation rates increased from 85.16% to 91.09% and from 49.39% to 63.07% in the two-phase, respectively. In contrast, without stripping, they were from 79.21% to 64.37% and from 50.26% to 53.15% in the single-phase, respectively. The microbial biodiversity was augmented via stripping, including norank_f__Spirochaetaceae, Petrimonas, Desulfurella and Blvii28_wastewater-sludge_group. Stripping operation enhanced the dissimilatory sulfate reduction, amino acid metabolism and possibly sulfate-dependent anaerobic ammonia oxidation (S-ANAMMOX). This study provides a promising strategy to improve sulfate reduction and reduce H₂S inhibition under a low COD/SO₄^2- ratio.

Keywords: Acidogenesis; COD/SO(4)(2−) ratio; Gas stripping; H(2)S; Sulfate reduction.