We report cutaneous pythiosis in 2 dogs in Italy that had recurrent exposure to the same freshwater habitat. Phylogenetic analysis placed the isolates within Pythium insidiosum complex cluster IV, corresponding to P. periculosum. In Italy, pythiosis should be considered in differential diagnoses by human and veterinary health professionals.
Keywords: Italy; One Health; Pythium insidiosum; Pythium periculosum; dermatology; dog; ecological niche; freshwater habitats; fungi; lake; parasites; pythiosis.