Moving bed bioreactors are efficient for the removal of nitrogen effluents by simultaneous nitrification and denitrification (SND), which is particularly challenging for effluents with low C/N ratio due to insufficient carbon available for denitrification. The addition of external organic carbon in such cases increases treatment costs. Endogenous degradation of biomass offers a cost-effective and persistent source of carbon. Internal carbon leads to low denitrification rates and optimizing the biomass content leads to a sustainable solution for treating such effluents. The present study aims to optimize the process of SND using endogenous carbon by investigating the effect of operational parameters like dissolved oxygen, influent ammonium (NH4-N) loading, hydraulic retention time (HRT) and carrier filling ratio (CFR). The synthetic wastewater used in the study does not inherently contain any organic carbon, thus indicating the responsible carbon for denitrification to be of internal origin. Using a novel carrier for multispecies biofilm development, initial NH4-N concentration variation showed an insignificant effect on SND efficiency. At higher loading, nitrite accumulation due to incomplete denitrification was observed. The effect of CFR was investigated in a range of 20-35%, beyond which non-homogeneous mixing was caused in the reactor. Under controlled DO and temperature of about 5 mg/L and 28 ± 2°C, respectively, an optimum filling ratio of 35% was finalised, resulting in an SND efficiency of 97.9% at 22 h HRT for high influent NH4-N concentration of 500 mg/L. Bacteriological species identification revealed the coexistence of nitrogen-fixing, aerobic nitrifying and anaerobic denitrifying bacteria in the biofilm growing on the biocarrier.Mechanism of SND occurring in aerobic moving bed bioreactors: Laboratory-scale aerobic Moving Bed Bioreactor setup for a continuous study evaluating the influence of operational parameters on SNDOccurrence of aerobic and anoxic zones in biofilm grown on the surface of novel carriers due to diffusional gradient of dissolved oxygen.
Keywords: MBBR; SND; biofilm process; low C/N ratio; nitrogen removal.