Study of hydrosalpinx on endometrial growth and expression of HOXA10mRNA and related factors

Taolan Li; Fan Lu; Chengrong Wu; Yi Ling Cai; La Yang; Hualei Cai

doi:10.1016/j.heliyon.2023.e17063

Study of hydrosalpinx on endometrial growth and expression of HOXA10mRNA and related factors

Heliyon. 2023 Jun 7;9(6):e17063. doi: 10.1016/j.heliyon.2023.e17063. eCollection 2023 Jun.

Authors

Taolan Li¹, Fan Lu², Chengrong Wu³, Yi Ling Cai⁴, La Yang², Hualei Cai²

Affiliations

¹ Department of Obstetrics and Gynecology, Guizhou Medical University, Guiyang, 550004, China.
² Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, China.
³ Guiyang Maternal and Child Health Care Hospital, Reproductive Center, Guiyang, 550004, China.
⁴ Bai Yun Hospital Affiliated to Guizhou Medical University, Department of Gynecology, Guiyang, 550004, China.

Abstract

Objective: The objective of the study is to extract the patient's endometrium at the time of proliferative stage using hydrosalpinx in order to culture the cells and decidualization induction in vitro. Further, the study is also intended at identifying the expression of HOXA10mRNA and related factors and understand the hydrosalpinx's impact upon the working mechanism of endometrial cells.

Methods: Once the extraction of the primary cells is over, the cells are cultured and other activities are performed such as the cell identification, CCK8 assay, cell decidua induction and HE staining. The researchers assessed the expression levels of HOXA10, IGFBP1 and avβ3 in either proliferation or secretion of the endometrium. This was accomplished using Western blot assay and real-time fluorescence quantitative PCR.

Results: The results confirmed that at the time of endometrial proliferation, there was a decline in the expression of HOXA10 as a result of tubal effusion influence. This affected its expression in the secretory stage i.e., corresponding function. Further, a significant decline was observed in the levels of HOXA10mRNA of endometrial cells that were subjected to continuous tubal effusion, post decidualization. It was found that during decidualization, if thetubal effusion is removed, it is possible to restore the expression of HOXA10mRNA to a certain extent, though it is not possible to reach the general endometrial level. So, in terms of clinical aspects, the expression of HOxa10 mRNA by the endometrial cells decreases significantly when blocking the hydrosalpinx.

Conclusions: Among hydrosalpinx patients, one of the major mechanisms that damage the endometrium was found to be the abnormal expression of HOXA10 followed by IGFBP1 and avβ3, its downstream genes. This further results in the implantation of the embryo as well. Though it is possible to gradually repair the damage after the removal of hydrosalpinx, the recovery is a time-consuming process.

Keywords: Endometrial receptivity; Homeobox protein A10 (HOXA10); Human insulin-like growth factor-binding protein1 (IGFBP1); Hydrosalpinx (HS); Hydrosalpinx fluid (HSF).