Critical Steps in DAPI and FISH Imaging of Chromosome Spread Preparations

Hans de Jong; José van de Belt; Paul Fransz

doi:10.1007/978-1-0716-3226-0_15

Critical Steps in DAPI and FISH Imaging of Chromosome Spread Preparations

Methods Mol Biol. 2023:2672:247-256. doi: 10.1007/978-1-0716-3226-0_15.

Authors

Hans de Jong¹, José van de Belt², Paul Fransz^{2

3}

Affiliations

¹ Wageningen University & Research, Laboratory of Genetics, Wageningen, The Netherlands. hans.dejong@wur.nl.
² Wageningen University & Research, Laboratory of Genetics, Wageningen, The Netherlands.
³ Swammerdam Institute of Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.

PMID: 37335481
DOI: 10.1007/978-1-0716-3226-0_15

Abstract

The final step in a long period of chromosome slide experiments is the publication of DAPI and multicolor fluorescence images. Quite often the result of published artwork is disappointing due to insufficient knowledge of image processing and presentation. In this chapter we describe some errors of fluorescence photomicrographs and how to avoid them. We include suggestions of processing chromosome images with simple examples of image processing in Photoshop^® or the like, without the need of complex knowledge of the software programs.

Keywords: DAPI; FISH; Fluorescence microscopy; Image processing; Imaging.

MeSH terms

Chromosomes
Diagnostic Imaging*
Image Processing, Computer-Assisted / methods
Indoles*

Substances

DAPI
Indoles