Frontiers and techniques in plant gene regulation

Tobias Jores; Morgan Hamm; Josh T Cuperus; Christine Queitsch

doi:10.1016/j.pbi.2023.102403

Frontiers and techniques in plant gene regulation

Curr Opin Plant Biol. 2023 Oct:75:102403. doi: 10.1016/j.pbi.2023.102403. Epub 2023 Jun 16.

Authors

Tobias Jores¹, Morgan Hamm², Josh T Cuperus³, Christine Queitsch⁴

Affiliations

¹ Department of Genome Sciences, University of Washington, Seattle, WA, USA. Electronic address: tobjores@uw.edu.
² Department of Genome Sciences, University of Washington, Seattle, WA, USA.
³ Department of Genome Sciences, University of Washington, Seattle, WA, USA. Electronic address: cuperusj@uw.edu.
⁴ Department of Genome Sciences, University of Washington, Seattle, WA, USA. Electronic address: queitsch@uw.edu.

PMID: 37331209
DOI: 10.1016/j.pbi.2023.102403

Abstract

Understanding plant gene regulation has been a priority for generations of plant scientists. However, due to its complex nature, the regulatory code governing plant gene expression has yet to be deciphered comprehensively. Recently developed methods-often relying on next-generation sequencing technology and state-of-the-art computational approaches-have started to further our understanding of the gene regulatory logic used by plants. In this review, we discuss these methods and the insights into the regulatory code of plants that they can yield.

Keywords: Cis-regulatory elements; DNA accessibility; Massively parallel reporter assay; Plant STARR-seq; Plant gene regulation; Single-cell sequencing; Single-molecule chromatin assay.

Publication types

Review
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Chromatin
Gene Expression Regulation, Plant / genetics
Genes, Plant*
High-Throughput Nucleotide Sequencing*

Substances

Chromatin