Genetic Diversity of Listeria Detected in the Production Environment of Meat Processing

O L Voronina; N N Ryzhova; E I Aksenova; M S Kunda; A V Kutuzova; T I Karpova; Yu K Yushina; I S Tartakovsky

doi:10.3103/S0891416823010111

Genetic Diversity of Listeria Detected in the Production Environment of Meat Processing

Mol Gen Microbiol Virol. 2023;38(1):21-28. doi: 10.3103/S0891416823010111. Epub 2023 Jun 11.

Authors

O L Voronina¹, N N Ryzhova¹, E I Aksenova¹, M S Kunda¹, A V Kutuzova¹, T I Karpova¹, Yu K Yushina², I S Tartakovsky¹

Affiliations

¹ Gamaleya Research Institute of Epidemiology and Microbiology, 123098 Moscow, Russia.
² Gorbatov Federal Research Center for Food Systems, 109316 Moscow, Russia.

Abstract

The safety of food production as concerns Listeria is the key to the sanitary wellbeing of manufactured products. Molecular-genetic methods for the analysis of Listeria, including whole-genome sequencing, are effective in monitoring persistent contaminants and in the epidemic investigation of cases of foodborne infections. They have been adopted in the European Union, United States, and Canada. In Russia, multilocus and whole-genome sequencing has proven itself in the analysis of clinical food isolates and Listeria from the environment. The objective of the study was molecular-genetic characterization of Listeria detected in the industrial environment of meat processing. To characterize the Listeria isolates, microbiological methods were used according to GOST (State Standard) 32031-2012, as well as multilocus sequencing, including the analysis of seven housekeeping genes and four virulence genes, as well as whole-genome sequencing. In swabs that were positive for the presence of Listeria spp. taken at two meat-processing plants in Moscow, Listeria monocytogenes constituted 81% and L. welshimeri 19%. The predominant genotype (Sequence Type, ST) of L. monocytogenes was ST8. The variety was supplemented with ST321, ST121, and ST2330 (CC9 (Clonal Complex 9)). L. welshimeri, which prevailed in the second production, was represented by ST1050 and ST2331. The genomic characteristics of L. welshimeri isolates confirmed that they have high adaptive capabilities both as concerns production conditions (including resistance to disinfectants) and the metabolic peculiarities of the gastrointestinal tract of animals. L. monocytogenes CC9 and CC121 are also correlated with food production in other countries. However, L. monocytogenes CC8 and CC321 can cause invasive listeriosis. The concordance in the internalin profile of the ST8 isolates from the industrial environment with the clinical isolates ST8 and ST2096 (CC8) is a cause for concern. The study showed the effectiveness of molecular-genetic methods in determining the diversity of Listeria detected in the production environment of meat processing, and laid the foundation for monitoring of persistent contaminants.

Keywords: Listeria monocytogenes; Listeria spp.; genotyping; meat-processing industry; whole-genome sequencing.

© Allerton Press, Inc. 2023, ISSN 0891-4168, Molecular Genetics, Microbiology and Virology, 2023, Vol. 38, No. 1, pp. 21–28. © Allerton Press, Inc., 2023.Russian Text © The Author(s), 2023, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2023, No. 1, pp. 24–31.