Comparative transcriptomic analysis reveals differences in MADS-box genes of different hypericum in Changbai Mountains

Xia Yunrui; Song Rui; Yang Xing; Zhao Zhe; Zhang Keqin; Zhang Nanyi

doi:10.1002/ece3.10196

Comparative transcriptomic analysis reveals differences in MADS-box genes of different hypericum in Changbai Mountains

Ecol Evol. 2023 Jun 13;13(6):e10196. doi: 10.1002/ece3.10196. eCollection 2023 Jun.

Authors

Xia Yunrui¹, Song Rui¹, Yang Xing¹, Zhao Zhe¹, Zhang Keqin², Zhang Nanyi¹

Affiliations

¹ Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science Jilin Agricultural University Changchun Jilin Province China.
² Jilin Agricultural Science and Technology University Jilin Jilin Province China.

Abstract

To explore the differences between the hypericum in the Changbai Mountains, we carried out a transcriptome analysis of two common hypericums in the area, which was Hypericum attenuatum Choisy and Hypericum longistylum Oliv. We screened the MADS-box genes to analyze divergence time and evolutionary selection expression, and determine their expression levels. The results showed that we detected 9287 differentially expressed genes in the two species, of which shared 6044 genes by the two species. Analysis of the selected MADS genes revealed that the species was in an environment adapted to its natural evolution. The divergence time estimation showed that the segregation of these genes in the two species was related to the changes of external environment and genome replication events. The results of relative expression showed that the later flowering period of Hypericum attenuatum Choisy was related to the higher expression of the SVP (SHORT VEGETATIVE PHASE) and the AGL12 (AGAMOUS LIKE 12), while the lower expression of the FUL (FRUITFULL).

Keywords: Hypericum; Hypericum attenuatum Choisy; Hypericum longistylum Oliv.; differential gene; divergence time; phylogeny analysis; positive selection.