Nanozyme-assisted ratiometric photoelectrochemical aptasensor over Cu2O nanocubes mediated photocurrent-polarity-switching based on S-scheme FeCdS@FeIn2S4 heterostructure

Fang-Fang Xin; Jin-Jin Xu; Jin Zhang; Ai-Jun Wang; Yadong Xue; Li-Ping Mei; Pei Song; Jiu-Ju Feng

doi:10.1016/j.bios.2023.115442

Nanozyme-assisted ratiometric photoelectrochemical aptasensor over Cu₂O nanocubes mediated photocurrent-polarity-switching based on S-scheme FeCdS@FeIn₂S₄ heterostructure

Biosens Bioelectron. 2023 Oct 1:237:115442. doi: 10.1016/j.bios.2023.115442. Epub 2023 Jun 7.

Authors

Fang-Fang Xin¹, Jin-Jin Xu¹, Jin Zhang¹, Ai-Jun Wang¹, Yadong Xue², Li-Ping Mei³, Pei Song⁴, Jiu-Ju Feng⁵

Affiliations

¹ College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Key Laboratory of Watershed Earth Surface Processes and Ecological Security, Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Zhejiang Normal University, Jinhua, 321004, China.
² Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China.
³ College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Key Laboratory of Watershed Earth Surface Processes and Ecological Security, Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Zhejiang Normal University, Jinhua, 321004, China. Electronic address: mei.lp@zjnu.edu.cn.
⁴ College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Key Laboratory of Watershed Earth Surface Processes and Ecological Security, Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Zhejiang Normal University, Jinhua, 321004, China; Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China. Electronic address: ppsong2020@outlook.com.
⁵ College of Geography and Environmental Sciences, College of Chemistry and Materials Science, Key Laboratory of Watershed Earth Surface Processes and Ecological Security, Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Zhejiang Normal University, Jinhua, 321004, China. Electronic address: jjfeng@zjnu.cn.

PMID: 37321042
DOI: 10.1016/j.bios.2023.115442

Abstract

The controllable modulation of the response mode is highly attractive for the construction of photoelectrochemical (PEC) sensors with improved sensitivity and anti-interference ability in complex real samples matrix. Here, we present a charming proof-of-concept ratiometric PEC aptasensor of enrofloxacin (ENR) analysis via the controllable signal transduction. Different with the traditional sensing mechanism, this ratiometric PEC aptasensor integrates the anodic PEC signal induced by PtCuCo nanozyme-catalyzed precipitation reaction and the polarity-switching cathodic PEC response mediated by Cu₂O nanocubes on S-scheme FeCdS@FeIn₂S₄ heterostructure. Taking advantages of the photocurrent-polarity-switching signal response model and the superior performance of the photoactive substrate material, the proposed ratiometric PEC aptasensor displays a good detection linear range for ENR analysis from 0.01 pg mL^-1 to 10 ng mL^-1, with a detection limit of 3.3 fg mL^-1. This study provides a general platform for detecting interested trace analytes in real samples and expands the diversity of sensing strategy design.

Keywords: Nanozyme; Photocurrent-polarity switching; Ratiometric photoelectrochemical aptasensor; S-scheme heterostructure.

MeSH terms

Aptamers, Nucleotide* / chemistry
Biosensing Techniques*
Electrochemical Techniques
Electrodes
Limit of Detection

Substances

Aptamers, Nucleotide