In this study, a liquid crystal (LC)-based assay for the real-time detection of tetracycline (Tc) was developed. The sensor was constructed by implementing an LC-based platform that utilized the chelating properties of Tc to target Tc metal ions. This design enabled Tc-dependent induction of changes in the optical image of the LC; these modifications could then be observed in real-time with the naked eye. The performance of the sensor in detecting Tc was investigated with various metal ions to identify the most effective metal ion for Tc detection. In addition, the selectivity of the sensor was evaluated using different antibiotics. A correlation between Tc concentration and the optical intensity of the LC optical images was established, which enabled the quantification of Tc concentrations. The proposed method can detect Tc concentrations with a detection limit as low as 2.67 pM. Tests were conducted on milk, honey, and serum samples, which demonstrated that the proposed assay is highly accurate and reliable. The high sensitivity and selectivity of the proposed method make it a promising tool for real-time Tc detection, with potential applications in fields ranging from biomedical research to agriculture.
Keywords: Antibiotics; Liquid crystal; Tetracycline.
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