MiR-29b detection in serum using an electrochemical biosensor for the early diagnosis of gestational diabetes

Jianhua Yan; Jiayu Wang; Hongjie Liu; Liwei Wang; Kefu Yu; Li Deng; Junyou Su; Hongfei Chen

doi:10.1016/j.ab.2023.115209

MiR-29b detection in serum using an electrochemical biosensor for the early diagnosis of gestational diabetes

Anal Biochem. 2023 Aug 1:674:115209. doi: 10.1016/j.ab.2023.115209. Epub 2023 Jun 11.

Authors

Jianhua Yan¹, Jiayu Wang¹, Hongjie Liu², Liwei Wang³, Kefu Yu⁴, Li Deng⁵, Junyou Su⁵, Hongfei Chen⁵

Affiliations

¹ Medical College, Guangxi University, Guangxi Nanning, 530004, China.
² School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.
³ School of Marine Sciences, Coral Reef Research Center of China, Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; State Key Laboratory of Featured Metal Materials and Life-cycle Safety for Composite Structures, Guangxi University, Nanning, 530004, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519080, China. Electronic address: wangliwei@gxu.edu.cn.
⁴ School of Marine Sciences, Coral Reef Research Center of China, Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519080, China.
⁵ Department of Obstetrics, The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530003, China.

PMID: 37311517
DOI: 10.1016/j.ab.2023.115209

Abstract

Gestational diabetes mellitus (GDM) is a severe perinatal condition with serious consequences for the growth and development of the mother and baby. MicroRNA-29b (miR-29b) is essential to the pathogenesis of GDM and can be used as a molecular biomarker for diagnosis. Given the limitations of current GDM screening technologies, there is a pressing need for a sensitive detection approach to evaluate serum miR-29b in GDM patients, thus aiding in disease treatment. In this study, an electrochemical biosensor Co₇Fe₃-CN nanoparticles (NPs) was developed. Using a duplex-specific nuclease (DSN) signal amplification strategy with a linear range of 1-10⁴ pM and a low detection limit of 0.79 pM, the ultra-sensitive detection and quantification of miR-29b were accomplished. The dependability and applicability of the developed biosensor were validated by the standard method of qRT-PCR, and the content of serum miR-29b in GDM patients was shown to be significantly lower than that in the control group (P = 0.03). Specifically, miR-29b concentrations could be detected from 2.0 to 7.5 and 2.4 to 7.3 pM using qRT-PCR and the biosensor, respectively. These similar results indicated that a biosensor based on miR-29b detection has the potential to be used in the point-of-care testing of GDM patients in clinical practice.

Keywords: Electrochemical biosensor; Gestational diabetes mellitus; MiR-29b biomarker; Molecular diagnosis; Point-of-care testing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques* / methods
Diabetes, Gestational* / diagnosis
Early Diagnosis
Female
Humans
MicroRNAs* / analysis
Nanoparticles*
Pregnancy

Substances

MicroRNAs