A G-quadruplex structure in microRNA interferes with messenger RNA recognition and controls gene expression

Kaixin Lyu; Chun Kit Kwok

doi:10.1039/d3cc01549a

A G-quadruplex structure in microRNA interferes with messenger RNA recognition and controls gene expression

Chem Commun (Camb). 2023 Jun 29;59(53):8230-8233. doi: 10.1039/d3cc01549a.

Authors

Kaixin Lyu¹, Chun Kit Kwok^{1

2}

Affiliations

¹ Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China.
² Shenzhen Research Institute of City University of Hong Kong, Shenzhen, China. ckkwok42@cityu.edu.hk.

PMID: 37309572
DOI: 10.1039/d3cc01549a

Abstract

We identify and characterize an RNA G-quadruplex (rG4) structure motif in the human microRNA 638 (hsa-miR-638). We investigate the formation and role of this rG4 in vitro and in cells, and reveal that it inhibits the miR-638 and MEF2C messenger RNA interaction and controls gene expression at the translational level.

MeSH terms

G-Quadruplexes*
Gene Expression
Humans
MicroRNAs* / genetics
RNA / chemistry
RNA, Messenger / genetics
RNA, Messenger / metabolism

Substances

RNA, Messenger
MicroRNAs
RNA
MIRN638 microRNA, human