The GmXTH1 gene improves drought stress resistance of soybean seedlings

Ye Zhang; Han-Zhu Zhang; Jia-Yu Fu; Ye-Yao Du; Jing Qu; Yang Song; Pi-Wu Wang

doi:10.1007/s11032-021-01258-5

The GmXTH1 gene improves drought stress resistance of soybean seedlings

Mol Breed. 2021 Dec 27;42(1):3. doi: 10.1007/s11032-021-01258-5. eCollection 2022 Jan.

Authors

Ye Zhang¹, Han-Zhu Zhang¹, Jia-Yu Fu¹, Ye-Yao Du¹, Jing Qu¹, Yang Song¹, Pi-Wu Wang¹

Affiliation

¹ Center for Plant Biotechnology, College of Agronomy, Jilin Agricultural University, 2888 Xincheng Street, Nanguan District, Changchun City, Jilin Province China.

Abstract

In order to study the role of GmXTH1 gene in alleviating drought stress, soybean seeds with GmXTH1 gene were transferred by T₄ treated with PEG6000 concentration of 0%, 5%, 10%, and 15% respectively. The germination potential, germination rate, germination index, and other indicators were measured. The results showed that the germination potential, germination rate, and germination index of OEA1 and OEA2 strains overexpressed in T₄ generation were significantly higher than those of the control material M18. After 0-day, 7-day, and 15-day drought stress, the analysis of seedling phenotypes and root-shoot of different T₄ generation transgenic soybean lines showed that under stress conditions, the growth of GmXTH1 overexpression material was generally better than that of the control material M18. The growth of GmXTH1 interference expression material was generally worse than that of the control material M18, with significant differences in plant phenotypes. The root system of GmXTH1 overexpressed material was significantly developed compared with that of the control material M18. The analysis of physiological and biochemical indexes showed that the relative water content and the activity of antioxidant enzymes (superoxide dismutase and peroxidase) of GmXTH1 transgenic soybean material were significantly higher than those of the control material M18, and the accumulation of malondialdehyde was lower under the same stress conditions at seedling stage. Fluorescence quantitative PCR assay showed that the relative expression of GmXTH1 gene in transgenic soybean was significantly increased after drought stress. The results showed that the overexpression of GmXTH1 could increase the total root length, surface area, total projection area, root volume, average diameter, total cross number, and total root tip number, thereby increasing the water intake and reducing the transpiration of water content in leaves, thus reducing the accumulation of MDA and producing more protective enzymes in a more effective and prompt way, reducing cell membrane damage to improve drought resistance of soybean.

Keywords: Drought stress; Germination index; GmXTH1 gene; Physiology and biochemistry; Soybean.