Potato Virus X Inactivation and Characterization

Yifeng Ma; Nicole F Steinmetz

doi:10.1007/978-1-0716-3222-2_15

Potato Virus X Inactivation and Characterization

Methods Mol Biol. 2023:2671:257-271. doi: 10.1007/978-1-0716-3222-2_15.

Authors

Yifeng Ma¹, Nicole F Steinmetz^{2

3

4

5

6

7

8}

Affiliations

¹ Department of NanoEngineering, University of California San Diego, La Jolla, CA, USA.
² Department of NanoEngineering, University of California San Diego, La Jolla, CA, USA. nsteinmetz@eng.ucsd.edu.
³ Department of Bioengineering, University of California San Diego, La Jolla, CA, USA. nsteinmetz@eng.ucsd.edu.
⁴ Department of Radiology, University of California San Diego, La Jolla, CA, USA. nsteinmetz@eng.ucsd.edu.
⁵ Center for Nano-ImmunoEngineering, University of California San Diego, La Jolla, CA, USA. nsteinmetz@eng.ucsd.edu.
⁶ Institute for Materials Discovery and Design, University of California San Diego, La Jolla, CA, USA. nsteinmetz@eng.ucsd.edu.
⁷ Moores Cancer Center, University of California San Diego, La Jolla, CA, USA. nsteinmetz@eng.ucsd.edu.
⁸ Center for Engineering in Cancer, Institute for Engineering in Medicine, University of California San Diego, La Jolla, CA, USA. nsteinmetz@eng.ucsd.edu.

PMID: 37308650
DOI: 10.1007/978-1-0716-3222-2_15

Abstract

The nucleoprotein components of plant viruses self-assemble into monodisperse, nanoscale structures with a high degree of symmetry and polyvalency. Of particular interest are the filamentous plant viruses which provide uniform high aspect ratio nanostructures-such structures remain challenging to obtain using purely synthetic approaches. Potato virus X (PVX) has drawn interest by the materials science community because of its filamentous structure measuring 515 × 13 nm; and both genetic engineering and chemical conjugation methods have been reported to impart new functionalities and develop PVX-based nanomaterials for applications in the health and materials sector. Toward environmentally safe materials-i.e., materials that are not infectious toward crops, such as potato, we reported methods to inactivate PVX. In this chapter, we describe the three methods to inactivate PVX and render it non-infectious toward plants, while maintaining structure and function.

Keywords: Formalin; Potato virus X (PVX); UV irradiation; Viral nanoparticles (VNPs); Virus inactivation; β-propiolactone.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Crops, Agricultural
Cytoskeleton
Plant Diseases
Potexvirus*
X Chromosome Inactivation