Pigeon TBK1 is involved in antiviral innate immunity by mediating IFN activation

Qi Shao; Feiyu Fu; Pei Zhu; Minzhi Xu; Jie Wang; Zhaofei Wang; Yaxian Yan; Hengan Wang; Jingjiao Ma; Yuqiang Cheng; Jianhe Sun

doi:10.1016/j.dci.2023.104758

Pigeon TBK1 is involved in antiviral innate immunity by mediating IFN activation

Dev Comp Immunol. 2023 Oct:147:104758. doi: 10.1016/j.dci.2023.104758. Epub 2023 Jun 10.

Authors

Qi Shao¹, Feiyu Fu¹, Pei Zhu¹, Minzhi Xu¹, Jie Wang¹, Zhaofei Wang¹, Yaxian Yan¹, Hengan Wang¹, Jingjiao Ma¹, Yuqiang Cheng², Jianhe Sun³

Affiliations

¹ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Agriculture Ministry Key Laboratory of Urban Agriculture (South), Shanghai, 200240, China.
² School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Agriculture Ministry Key Laboratory of Urban Agriculture (South), Shanghai, 200240, China. Electronic address: wyycyq@sjtu.edu.cn.
³ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Agriculture Ministry Key Laboratory of Urban Agriculture (South), Shanghai, 200240, China. Electronic address: sunjhe@sjtu.edu.cn.

PMID: 37307868
DOI: 10.1016/j.dci.2023.104758

Abstract

TANK-binding kinase 1 (TBK1), a noncanonical member of the inhibitor-kappaB kinases (IKKs) family, plays a vital role in regulating type-I interferon (IFN) production in mammals and birds. We cloned pigeon TBK1 (PiTBK1) and conducted bioinformatics analyses to compare the protein homology of TBK1 from different species. Overexpression of PiTBK1 in DF-1 cells induced the activation of IFN-β, and this activation positively correlated with the dosage of transfected PiTBK1 plasmids. In pigeon embryonic fibroblasts (PEFs) cells, it does the same. And the STK and Ubl domain are essential for IFN-β activation. Consistent with the previous results, when PiTBK1 expressed more, NDV replication was lower. Our results suggest that PiTBK1 is an important regulator of IFNs and plays a pivotal role in antiviral innate immunity in pigeon.

Keywords: Innate immunity; Interferon; Newcastle disease virus; Pigeon; TANK-Binding kinase 1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antiviral Agents*
Columbidae*
Immunity, Innate
Interferon Regulatory Factor-3 / metabolism
Mammals
Phosphorylation
Signal Transduction

Substances

Antiviral Agents
Interferon Regulatory Factor-3