Endophytic fungus Biscogniauxia petrensis produces antibacterial substances

Long Han; Wen Zheng; Zhangjiang He; Shengyan Qian; Xiaoya Ma; Jichuan Kang

doi:10.7717/peerj.15461

Endophytic fungus Biscogniauxia petrensis produces antibacterial substances

PeerJ. 2023 Jun 7:11:e15461. doi: 10.7717/peerj.15461. eCollection 2023.

Authors

Long Han^{1

2}, Wen Zheng^{1

2}, Zhangjiang He², Shengyan Qian^{1

2}, Xiaoya Ma^{2

3}, Jichuan Kang²

Affiliations

¹ College of Life Sciences, Guizhou University, Guiyang, China.
² Engineering Research Center of the Utilization for Characteristic Bio-pharmaceutical Resources in Southwestern, Ministry of Education, Guizhou University, Guiyang, China.
³ School of Science, Mae Fah Luang University, Chiang Rai, Thailand.

Abstract

Widespread drug resistance and limited antibiotics challenge the treatment of pathogenic bacteria, which leads to a focus on searching for new antimicrobial lead compounds. We found the endophytic fungus Biscogniauxia petrensis MFLUCC14-0151 from the medicinal plant Dendrobium harveyanum had antibacterial activity for the first time. This work aimed to reveal the capacity of Biscogniauxia petrensis MFLUCC14-0151 against foodborne pathogenic bacteria and identify its bioactive substances. Bioassay-guided isolation led to the discovery of six infrequent active monomers, including (10R)-Xylariterpenoid B (1), Xylariterpenoid C (2), Tricycloalternarene 1b (3), Tricycloalternarene 3b (4), Funicin (5) and Vinetorin (6) from MFLUCC14-0151 for the first time. The results of antibacterial tests showed that (10R)-Xylariterpenoid B and Xylariterpenoid C exhibited inhibitory activities against Streptococcus agalactiae with MIC values ranging from 99.21 to 100.00 μM, and against Streptococcus aureus with MIC values ranging from 49.60 to 50.00 μM. Tricycloalternarene 1b and Tricycloalternarene 3b showed inhibitory effects on Streptococcus agalactiae with MIC values ranging from 36.13 to 75.76 μM. Unexpectedly, Funicin and Vinetorin exhibited remarkable antagonistic activities against Streptococcus agalactiae with MIC values of 10.35 and 10.21 μM, respectively, and against Streptococcus aureus with MIC values of 5.17 and 20.42 μM, respectively. In conclusion, we suggest that the isolated compounds Funicin and Vinetorin may be promising lead compounds for natural antibacterial agents.

Keywords: Antibacterial substance; Biscogniauxia petrensis; Endophytic fungus; Foodborne pathogen; Funicin; Medicinal plant; Vinetorin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Ascomycota*
Biological Assay
Streptococcus agalactiae

Substances

Anti-Bacterial Agents

Supplementary concepts

Biscogniauxia petrensis

Grants and funding

This work was supported by the National Natural Science Foundation of China (No. 31670027, 32170019, 32160667 and 31901947). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.