Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway

Ruidong Ge; Bin Ruan; Shengxuan Chen; Shuo Bai; Qi Gao; Anran Dong

Radial extracorporeal shock wave therapy alleviates acute inflammation of human primary tenocytes through the integrin-FAK-p38MAPK pathway

Am J Transl Res. 2023 May 15;15(5):3229-3239. eCollection 2023.

Authors

Ruidong Ge^{1

2}, Bin Ruan¹, Shengxuan Chen³, Shuo Bai⁴, Qi Gao¹, Anran Dong¹

Affiliations

¹ School of Sports Medicine and Rehabilitation, Beijing Sport University Beijing 100084, China.
² Department of Rehabilitation Medicine, China-Japan Friendship Hospital Beijing 100029, China.
³ Department of Rehabilitation Medicine, The Gulou Hospital of Traditional Chinese Medicine Beijing 100009, China.
⁴ Department of Rehabilitation Medicine, Beijing Chaoyang District Taiyanggong Community Health Service Center Beijing 100028, China.

PMID: 37303680
PMCID: PMC10251031

Abstract

Objectives: To explore whether radial extracorporeal shock wave therapy (rESWT) can alleviate acute inflammation of human primary tenocytes by the integrin-focal adhesion kinase (FAK)-p38 mitogen-activated protein kinase (MAPK) pathway.

Methods: Western blotting was used to evaluate the changes in the integrin-FAK-p38MAPK signaling pathway mediated by rESWT using specific antibodies targeting the phosphorylation sites of intracellular signal pathway proteins.

Results: rESWT up-regulated FAK phosphorylation and down-regulated p38MAPK phosphorylation levels in a tumor necrosis factor (TNF)-α-induced acute inflammation model of human primary tenocytes. Pretreatment with an integrin inhibitor significantly reduced rESWT-mediated downregulation of p38MAPK phosphorylation and attenuated its reversal effect on the increased secretion of pro-inflammatory cytokines in TNF-α-induced human primary tenocytes.

Conclusions: Our results imply that rESWT may partially alleviate acute inflammation in human primary tenocytes through the integrin-FAK-p38MAPK pathway.

Keywords: Inflammation; integrin; mitogen-activated protein kinases; shockwave; tenocyte.