A portable, high-throughput real-time quantitative PCR device for point-of-care testing

Juxin Yin; Jizhi Tong; Jiale Li; Guangye Shao; Bo Xie; Jianjian Zhuang; Gang Bi; Ying Mu

doi:10.1016/j.ab.2023.115200

A portable, high-throughput real-time quantitative PCR device for point-of-care testing

Anal Biochem. 2023 Aug 1:674:115200. doi: 10.1016/j.ab.2023.115200. Epub 2023 Jun 9.

Authors

Juxin Yin¹, Jizhi Tong¹, Jiale Li², Guangye Shao³, Bo Xie³, Jianjian Zhuang⁴, Gang Bi⁵, Ying Mu⁶

Affiliations

¹ School of Information and Electrical Engineering, Hangzhou City University, Hangzhou, Zhejiang Province, 310015, China.
² Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027, China.
³ Hang Zhou Techway Gene CO.LTD, Zhejiang Province, 310015, China.
⁴ Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, Cancer Center, Zhejiang University School of Medicine, Hangzhou, 310006, China. Electronic address: zhuangjianjian1234@163.com.
⁵ School of Information and Electrical Engineering, Hangzhou City University, Hangzhou, Zhejiang Province, 310015, China. Electronic address: big@zucc.edu.cn.
⁶ Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province, 310027, China. Electronic address: muying@zju.edu.cn.

PMID: 37302776
DOI: 10.1016/j.ab.2023.115200

Abstract

Nucleic acids detection has become essential in the identification of many infectious diseases and tumors. Conventional qPCR instruments are not suitable for point-of-care Moreover, current miniaturized nucleic acid detection equipment has limited throughput and multiplex detection capabilities, typically allowing the detection of a limited number of samples. Here, we present an affordable, portable, and high-throughput nucleic acid detection device for point-of-care detection. This portable device is approximately 220 × 165 × 140 mm in size and about 3 kg in weight. It can provide stable and accurate temperature control and analyze two fluorescent signals (FAM and VIC) and run 16 samples simultaneously. As a proof of concept, we used the two purified DNA samples from Bordetella pertussis and Canine parvovirus and the results showed good linearity and coefficient of variation. Moreover, this portable device can detect as low as 10 copies and has good specificity. Therefore, our device can provide advantages in real-time diagnosis of high-throughput nucleic acid detection in the field, especially for resource-limited conditions.

Keywords: High-throughput; Nucleic acids detection; Point-of-care; qPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA
Nucleic Acid Amplification Techniques / methods
Point-of-Care Systems*
Point-of-Care Testing*
Real-Time Polymerase Chain Reaction / methods
Sensitivity and Specificity

Substances

DNA