The Complete Chloroplast Genome Sequence of Staphylea holocarpa (Staphyleaceae)

Mol Biotechnol. 2024 Jun;66(6):1458-1463. doi: 10.1007/s12033-023-00780-5. Epub 2023 Jun 10.

Abstract

Staphylea holocarpa (Hemsley 1895) is an ornamental deciduous shrub or tree in the family Staphyleaceae. As the shortage of the wild resources, S. holocarpa is also a rare plant. The revelation of the species origin and evolution progress and the relation. Therefore, the S. holocarpa complete chloroplast genome sequence was completed and characterized by de novo assembly. The cp genome length of S. holocarpa was 160,461 bp and it has a typical quadripartite structure, consisted of an 89,760 bp large single-copy region and a 18,639 bp small single-copy region, which were divided by two inverted repeat regions of 26,031 bp. After genome annotation, it comes to 130 genes that were predicted, which includes 85, 8, and 37 encoded proteins, rRNA, and tRNA, respectively. A phylogenetic analysis has shown that the S. holocarpa cp genome is related to the Staphylea trifolia. This work will be useful for further population genomic and phylogenetic studies of S. holocarpa.

Keywords: Staphylea holocarpa; Chloroplast genome; Phylogenetic analysis; Staphyleaceae.

MeSH terms

  • Genome, Chloroplast*
  • Molecular Sequence Annotation
  • Phylogeny*
  • RNA, Transfer / genetics
  • Sequence Analysis, DNA
  • Whole Genome Sequencing