Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling

Christine M O'Keefe; Tza-Huei Jeff Wang

doi:10.1007/978-1-0716-3271-0_22

Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling

Methods Mol Biol. 2023:2679:315-322. doi: 10.1007/978-1-0716-3271-0_22.

Authors

Christine M O'Keefe¹, Tza-Huei Jeff Wang²

Affiliations

¹ Johns Hopkins University, Whiting School of Engineering, Department of Biomedical Engineering, Baltimore, MD, USA.
² Johns Hopkins University, Whiting School of Engineering, Department of Biomedical Engineering, Baltimore, MD, USA. thwang@jhu.edu.

PMID: 37300626
DOI: 10.1007/978-1-0716-3271-0_22

Abstract

Trace amounts of cell-free DNA containing cancer-specific biomarkers can be found in blood plasma. Detection of these biomarkers holds tremendous potential for applications such as noninvasive cancer diagnostics and therapeutic monitoring. However, such DNA molecules are extremely rare, and a typical patient blood sample may only contain a few copies. Here we describe the fabrication and operation of a microfluidic device to efficiently trap single DNA molecules into chambers for detection of tumor-specific biomarkers through a passive, geometric manipulation strategy.

Keywords: Circulating DNA; Digital PCR; Digital melt; Heterogeneity; Microfluidic DNA capture efficiency; Molecule-by-molecule profiling; Passive microfluidics.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Biomarkers, Tumor
DNA
Humans
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques*
Microfluidics
Neoplasms*

Substances

DNA
Biomarkers, Tumor

Abstract

Publication types

MeSH terms

Substances

Grants and funding