This experiment was conducted to evaluate the growth performance, growth regulating factors, and liver morphology of chicks hatched from egg-laying breeding hens dietary supplemented with additives (β-carotene). Hy-line breeding hens were allocated into three groups with three replicates/group. The dietary treatments were as follows: basal diet as a control (Con), basal diet supplemented with 120 (βc-L) or 240 (βc-H) mg/kg of β-carotene diet. After 6 weeks, the eggs were collected and incubated. The hatched chicks were fed the same diet. The results showed that chicks in the βc-L group increased in body weight at 21 days (p < 0.01). At 42 days, chicks in the βc-H group showed a significant increase in tibia length (p < 0.05). The liver index increased in the βc-L and βc-H groups at 7 days (p < 0.05). Serum HGF (7, 14, 21, and 42 days) and leptin (14 days) were significantly increased in the group supplemented with βc. Hepatic GHR (14 days), IGF-1R (14 days), and LEPR (21 days) mRNA expression were significantly increased. In addition, there was an increase in PCNA-positive cells in the liver of chicks in the βc group. In conclusion, the addition of β-carotene to the diet of laying breeder hens was more advantageous in terms of growth performance and liver development of the offspring.
Keywords: Beta-carotene additives; growth regulator; hepatocyte proliferation; offspring chicks.