Genome-Wide Analysis of WRKY Transcription Factors Involved in Abiotic Stress and ABA Response in Caragana korshinskii

Jinhua Liu; Guojing Li; Ruigang Wang; Guangxia Wang; Yongqing Wan

doi:10.3390/ijms24119519

Genome-Wide Analysis of WRKY Transcription Factors Involved in Abiotic Stress and ABA Response in Caragana korshinskii

Int J Mol Sci. 2023 May 30;24(11):9519. doi: 10.3390/ijms24119519.

Authors

Jinhua Liu¹, Guojing Li¹, Ruigang Wang¹, Guangxia Wang¹, Yongqing Wan¹

Affiliation

¹ Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China.

Abstract

The WRKY transcription factor family plays a vital role in plant development and environmental response. However, the information of WRKY genes at the genome-wide level is rarely reported in Caragana korshinskii. In this study, we identified and renamed 86 CkWRKY genes, which were further classified into three groups through phylogenetic analysis. Most of these WRKY genes were clustered and distributed on eight chromosomes. Multiple sequence alignment revealed that the conserved domain (WRKYGQK) of the CkWRKYs was basically consistent, but there were also six variation types (WRKYGKK, GRKYGQK, WRMYGQK, WRKYGHK, WKKYEEK and RRKYGQK) that appeared. The motif composition of the CkWRKYs was quite conservative in each group. In general, the number of WRKY genes gradually increased from lower to higher plant species in the evolutionary analysis of 28 species, with some exceptions. Transcriptomics data and RT-qPCR analysis showed that the CkWRKYs in different groups were involved in abiotic stresses and ABA response. Our results provided a basis for the functional characterization of the CkWRKYs involved in stress resistance in C. korshinskii.

Keywords: Caragana korshinskii; WRKY; evolutionary analysis; genome-wide; transcriptome.

MeSH terms

Caragana* / genetics
Gene Expression Regulation, Plant
Multigene Family
Phylogeny
Plant Proteins / genetics
Plant Proteins / metabolism
Stress, Physiological / genetics
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Transcription Factors
Plant Proteins

Grants and funding

This work was financially supported by The Major Project of Inner Mongolia Natural Science Foundation (2019ZD05); National Natural Science Foundation of China (No. 31860217); Special Project in Major Science and Technology Program of Hohhot (2022-S-M-1-3); Development Fund from the Central Government Guiding Local Science and Technology Program (2022ZY0008); Research Team of Inner Mongolia Agricultural University (BR22-13-05); The University Scientific and Technological Innovation Team Project of Inner Mongolia (NMGIRT2222).