Purification and structural analysis of a novel polysaccharide from Rehmannia Radix Praeparata

Chem Biol Drug Des. 2023 Sep;102(3):514-522. doi: 10.1111/cbdd.14261. Epub 2023 Jun 7.

Abstract

In this paper, the purification, structure, and antioxidant activity of Rehmannia Radix Praeparata polysaccharide (RRPP) were studied. The RRPP was separated using DEAE-52 cellulose and Sephadex G-100. The RRPP consisted of xylose, glucose, rhamnose, galactose, and mannose in ratios of 10.64:5.58:3.52:1.39:1.0. No protein was detected in the RRPP fraction, and the molecular weight of RRPP was about 1.75 × 106 Da. The basic skeleton information was obtained using periodic acid oxidation-Smith degradation, and RRPP contained 1→, 1 → 2, 1 → 3, 1 → 4, 1 → 2,6, 1 → 4,6 or 1 → 6, 1 → 2,3, 1 → 2,3,4, and other glycosidic bonds. Fourier transform infrared spectroscopy also showed that RRPP has both α- and β-glycosidic bonds. The in vitro antioxidant activity test showed that RRPP could potentialize scavenging effect on ABTS+· and its scavenging rate was 91.3%.

Keywords: Rehmannia Radix Praeparata polysaccharide; antioxidant activity; purification; separation; structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants* / chemistry
  • Antioxidants* / pharmacology
  • Galactose
  • Molecular Weight
  • Oxidation-Reduction
  • Polysaccharides / chemistry
  • Rehmannia* / chemistry
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Antioxidants
  • Polysaccharides
  • Galactose